%0 Journal Article %1 Vogelsang2008 %A Vogelsang, Jan %A Kasper, Robert %A Steinhauer, Christian %A Person, Britta %A Heilemann, Mike %A Sauer, Markus %A Tinnefeld, Philip %C PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY %D 2008 %I WILEY-V C H VERLAG GMBH %J ANGEWANDTE CHEMIE-INTERNATIONAL EDITION %K mike sauer %N 29 %P 5465-5469 %T A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes %U http://dx.doi.org/10.1002/anie.200801518 %V 47 %X On the ROXS: Photobleaching represents one of the main limitations of modern fluorescence microscopy. A reducing and oxidizing system (ROXS) has been introduced that recovers triplet states as well as charge-separated states through electron-transfer reactions (see picture). Thus the blinking and photobleaching of fluorophores is strikingly reduced.

@article{Vogelsang2008, abstract = {On the ROXS: Photobleaching represents one of the main limitations of modern fluorescence microscopy. A reducing and oxidizing system (ROXS) has been introduced that recovers triplet states as well as charge-separated states through electron-transfer reactions (see picture). Thus the blinking and photobleaching of fluorophores is strikingly reduced.}, added-at = {2011-03-01T10:57:13.000+0100}, address = {PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY}, affiliation = {Tinnefeld, P (Reprint Author), Univ Munich, Ctr NanoSci, Amalienstr 54, D-80799 Munich, Germany. {[}Vogelsang, Jan; Steinhauer, Christian; Person, Britta; Tinnefeld, Philip] Univ Munich, Ctr NanoSci, D-80799 Munich, Germany. {[}Kasper, Robert; Heilemann, Mike; Sauer, Markus] Univ Bielefeld, D-33615 Bielefeld, Germany.}, author = {Vogelsang, Jan and Kasper, Robert and Steinhauer, Christian and Person, Britta and Heilemann, Mike and Sauer, Markus and Tinnefeld, Philip}, author-email = {Philip.Tinnefeld@lmu.de}, biburl = {https://www.bibsonomy.org/bibtex/234da7f1e2a9895379193a53350e75721/reichert}, cited-references = {BRUCHEZ M, 1998, SCIENCE, V281, P2013. CHIBISOV AK, 1977, J PHOTOCHEM, V6, P199. COTLET M, 2004, P NATL ACAD SCI USA, V101, P14343, DOI 10.1073/pnas.0406119101. COTLET M, 2005, J AM CHEM SOC, V127, P9760, DOI 10.1021/ja042656o. EGGELING C, 2005, CHEMPHYSCHEM, V6, P791, DOI 10.1002/cphc.200400509. EGGELING C, 2006, J PHYS CHEM A, V110, DOI 10.1021/jp054581w. GIEPMANS BNG, 2006, SCIENCE, V312, P217, DOI 10.1126/science.1124618. HA T, 2001, METHODS, V25, P78. HARADA Y, 1990, J MOL BIOL, V216, P49. HEILEMANN M, 2005, J AM CHEM SOC, V127, P3801, DOI 10.1021/ja044686x. HEILEMANN M, 2006, J AM CHEM SOC, V128, P16864, DOI 10.1021/ja065585x. HELL SW, 2007, SCIENCE, V316, P1153, DOI 10.1126/science.1137395. HOHNG S, 2007, SCIENCE, V318, P279, DOI 10.1126/science.1146113. HOLMAN MW, 2003, J AM CHEM SOC, V125, P12649, DOI 10.1021/ja0343104. HOOGENBOOM JP, 2005, PHYS REV LETT, V95, ARTN 097401. HUANG ZX, 2005, J AM CHEM SOC, V127, P8064, DOI 10.1021/ja050050+. KASPER R, 2007, P SPIE INT SOC OPT E, V6633, UNSP 66331Z1. KAVARNOS GJ, 1993, FUNDAMENTALS PHOTOIN. KIEL A, 2007, ANGEW CHEM INT EDIT, V46, P5049, DOI 10.1002/anie.200604965. KIEL A, 2007, ANGEW CHEM, V119, P3427. KONG XX, 2007, J AM CHEM SOC, V129, P4643, DOI 10.1021/ja068002s. LAMBERT CR, 1997, PHOTOCHEM PHOTOBIOL, V66, P15. LIPHARDT B, 1981, OPT COMMUN, V38, P207. LIPHARDT B, 1983, OPT COMMUN, V48, P129. LIU RC, 2003, J PHYS CHEM A, V107, P6522, DOI 10.1021/jp034239o. LU HP, 1997, J PHYS CHEM B, V101, P2753. MCKINNEY SA, 2003, NAT STRUCT BIOL, V10, P93, DOI 10.1038/nsb883. MICHALET X, 2005, SCIENCE, V307, P538, DOI 10.1126/science.1104274. RASNIK I, 2006, NAT METHODS, V3, P891, DOI 10.1038/NMETH934. REHM D, 1970, ISR J CHEM, V8, P259. SCHAFER FP, 1973, DYE LASERS. SCHUSTER J, 2005, OPT SPECTROSC+, V98, P712. TINNEFELD P, 2003, J PHYS CHEM A, V107, P323, DOI 10.1021/jp026565u. TINNEFELD P, 2005, ANGEW CHEM INT EDIT, V44, P2642, DOI 10.1002/anie.200300647. TINNEFELD P, 2005, ANGEW CHEM, V117, P2698. VOSCH T, 2003, J PHYS CHEM A, V107, P6920, DOI 10.1021/jp034906d. VOSCH T, 2007, P NATL ACAD SCI USA, V104, P12616, DOI 10.1073/pnas.0610677104. WIDENGREN J, 2000, J PHYS CHEM A, V104, P6416. WIDENGREN J, 2007, J PHYS CHEM A, V111, P429, DOI 10.1021/jp0646325. YEOW EKL, 2006, J PHYS CHEM A, V110, P1726, DOI 10.1021/jp055496r. YUSTE R, 2005, NAT METHODS, V2, P902. ZONDERVAN R, 2003, J PHYS CHEM A, V107, P6770, DOI 10.1021/jp034723r.}, doc-delivery-number = {325PQ}, groups = {public}, interhash = {2f522c86ea8eca6e59f74f356b72411f}, intrahash = {34da7f1e2a9895379193a53350e75721}, issn = {1433-7851}, journal = {ANGEWANDTE CHEMIE-INTERNATIONAL EDITION}, journal-iso = {Angew. Chem.-Int. Edit.}, keywords = {mike sauer}, keywords-plus = {INTERFACIAL ELECTRON-TRANSFER; SINGLE-MOLECULE SPECTROSCOPY; RESONANCE ENERGY-TRANSFER; BACK-ISOMERIZATION; RHODAMINE 6G; LASER-DYES; KINETICS; TRIPLET; STATE; MICROSCOPY}, language = {English}, number = 29, number-of-cited-references = {42}, pages = {5465-5469}, publisher = {WILEY-V C H VERLAG GMBH}, subject-category = {Chemistry, Multidisciplinary}, times-cited = {56}, timestamp = {2011-03-10T14:07:00.000+0100}, title = {A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes}, type = {Article}, unique-id = {ISI:000257601200042}, url = {http://dx.doi.org/10.1002/anie.200801518}, username = {reichert}, volume = 47, year = 2008 }