%0 Journal Article %1 RN1235 %A Biela, A. %A Grote, K. %A Otto, B. %A Hoth, S. %A Hedrich, R. %A Kaldenhoff, R. %D 1999 %J Plant Journal %K activity channel myOwn water %N 5 %P 565-570 %R DOI 10.1046/j.1365-313X.1999.00474.x %T The plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol %U /brokenurl#<Go to ISI>://WOS:000081233900011 %V 18 %X A new aquaporin from Nicotiana tabacum (cv. Samsun) was characterized. It shares sequence homology to the Arabidopsis thaliana PIP1 protein family. By two-phase partitioning and immunoblot analysis, plasma membrane localization could be demonstrated. The corresponding mRNA is highly abundant in roots and flowers, while it is rarely expressed in leaves and stems. Functional expression in Xenopus oocytes revealed that NtAQP1 can mediate glycerol transport in addition to water flow. However, NtAQP1 is impermeable for Na+, K+ and Cl- ions. The water permeability and selectivity could not be modulated by addition of mercurials or the activity of cAMP-dependent protein kinases.

@article{RN1235, abstract = {A new aquaporin from Nicotiana tabacum (cv. Samsun) was characterized. It shares sequence homology to the Arabidopsis thaliana PIP1 protein family. By two-phase partitioning and immunoblot analysis, plasma membrane localization could be demonstrated. The corresponding mRNA is highly abundant in roots and flowers, while it is rarely expressed in leaves and stems. Functional expression in Xenopus oocytes revealed that NtAQP1 can mediate glycerol transport in addition to water flow. However, NtAQP1 is impermeable for Na+, K+ and Cl- ions. The water permeability and selectivity could not be modulated by addition of mercurials or the activity of cAMP-dependent protein kinases.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Biela, A. and Grote, K. and Otto, B. and Hoth, S. and Hedrich, R. and Kaldenhoff, R.}, biburl = {https://www.bibsonomy.org/bibtex/2352710859a4a75aef7363a1e43b22319/rainerhedrich_2}, doi = {DOI 10.1046/j.1365-313X.1999.00474.x}, interhash = {39f18d3ab6bcaaf4aa9ee034417f7037}, intrahash = {352710859a4a75aef7363a1e43b22319}, issn = {0960-7412}, journal = {Plant Journal}, keywords = {activity channel myOwn water}, note = {212ty Times Cited:188 Cited References Count:32}, number = 5, pages = {565-570}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {The plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000081233900011}, volume = 18, year = 1999 }