%0 Journal Article %1 RN1188 %A Deeken, R. %A Ivashikina, N. %A Czirjak, T. %A Philippar, K. %A Becker, D. %A Ache, P. %A Hedrich, R. %D 2003 %J Plant Journal %K arabidopsis myOwn %N 6 %P 778-787 %R DOI 10.1046/j.1365-313X.2003.01766.x %T Tumour development in involves the -like K channels AKT1 and AKT2/3 %U /brokenurl#<Go to ISI>://WOS:000183443000004 %V 34 %X After completion of the Arabidopsis genome-sequencing programme, crown galls induced by Agrobacterium tumefaciens may become a model system to study plant tumour development. The molecular mechanisms of nutrient supply to support tumour growth and development are still unknown. In this study, we have identified a unique profile of Shaker -like potassium channels in agrobacteria-induced Arabidopsis tumours. Comparing the gene expression pattern of rapidly growing tumours with that of non-infected tissues, we found the suppression of shoot in favour of root-specific K+ channels. Among these, the upregulation of AKT1 and AtKC1 and the suppression of AKT2/3 and GORK were most pronounced. As a consequence, K+ uptake and accumulation were elevated in the tumour (163 mm) compared to control tissues (92 mm). Patch clamp studies on tumour protoplasts identified a population expressing the electrical properties of the AKT1 K+ channel. Furthermore, plants lacking a functional AKT1 or the AKT2/3 phloem K+ channel gene did not support tumour growth. This indicates that the delivery of potassium by AKT1 and the direction of assimilates, triggered by AKT2/3, are essential for tumour growth.

@article{RN1188, abstract = {After completion of the Arabidopsis genome-sequencing programme, crown galls induced by Agrobacterium tumefaciens may become a model system to study plant tumour development. The molecular mechanisms of nutrient supply to support tumour growth and development are still unknown. In this study, we have identified a unique profile of Shaker -like potassium channels in agrobacteria-induced Arabidopsis tumours. Comparing the gene expression pattern of rapidly growing tumours with that of non-infected tissues, we found the suppression of shoot in favour of root-specific K+ channels. Among these, the upregulation of AKT1 and AtKC1 and the suppression of AKT2/3 and GORK were most pronounced. As a consequence, K+ uptake and accumulation were elevated in the tumour (163 mm) compared to control tissues (92 mm). Patch clamp studies on tumour protoplasts identified a population expressing the electrical properties of the AKT1 K+ channel. Furthermore, plants lacking a functional AKT1 or the AKT2/3 phloem K+ channel gene did not support tumour growth. This indicates that the delivery of potassium by AKT1 and the direction of assimilates, triggered by AKT2/3, are essential for tumour growth.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Deeken, R. and Ivashikina, N. and Czirjak, T. and Philippar, K. and Becker, D. and Ache, P. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/210b4af32a5570f54c68f5904c4bd1f98/rainerhedrich_2}, doi = {DOI 10.1046/j.1365-313X.2003.01766.x}, interhash = {41132e56e50e3118bbc43280281604fa}, intrahash = {10b4af32a5570f54c68f5904c4bd1f98}, issn = {0960-7412}, journal = {Plant Journal}, keywords = {arabidopsis myOwn}, note = {688nw Times Cited:31 Cited References Count:52}, number = 6, pages = {778-787}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Tumour development in involves the -like K channels AKT1 and AKT2/3}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000183443000004}, volume = 34, year = 2003 }