%0 Journal Article %1 Wootton:2002:Nature:12124623 %A Wootton, J C %A Feng, X %A Ferdig, M T %A Cooper, R A %A Mu, J %A Baruch, D I %A Magill, A J %A Su, X Z %D 2002 %J Nature %K malaria spatial %N 6895 %P 320-323 %R 10.1038/nature00813 %T Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum %U http://www.ncbi.nlm.nih.gov/pubmed/12124623?dopt=Abstract %V 418 %X Widespread use of antimalarial agents can profoundly influence the evolution of the human malaria parasite Plasmodium falciparum. Recent selective sweeps for drug-resistant genotypes may have restricted the genetic diversity of this parasite, resembling effects attributed in current debates to a historic population bottleneck. Chloroquine-resistant (CQR) parasites were initially reported about 45 years ago from two foci in southeast Asia and South America, but the number of CQR founder mutations and the impact of chlorquine on parasite genomes worldwide have been difficult to evaluate. Using 342 highly polymorphic microsatellite markers from a genetic map, here we show that the level of genetic diversity varies substantially among different regions of the parasite genome, revealing extensive linkage disequilibrium surrounding the key CQR gene pfcrt and at least four CQR founder events. This disequilibrium and its decay rate in the pfcrt-flanking region are consistent with strong directional selective sweeps occurring over only approximately 20-80 sexual generations, especially a single resistant pfcrt haplotype spreading to very high frequencies throughout most of Asia and Africa. The presence of linkage disequilibrium provides a basis for mapping genes under drug selection in P. falciparum.

@article{Wootton:2002:Nature:12124623, abstract = {Widespread use of antimalarial agents can profoundly influence the evolution of the human malaria parasite Plasmodium falciparum. Recent selective sweeps for drug-resistant genotypes may have restricted the genetic diversity of this parasite, resembling effects attributed in current debates to a historic population bottleneck. Chloroquine-resistant (CQR) parasites were initially reported about 45 years ago from two foci in southeast Asia and South America, but the number of CQR founder mutations and the impact of chlorquine on parasite genomes worldwide have been difficult to evaluate. Using 342 highly polymorphic microsatellite markers from a genetic map, here we show that the level of genetic diversity varies substantially among different regions of the parasite genome, revealing extensive linkage disequilibrium surrounding the key CQR gene pfcrt and at least four CQR founder events. This disequilibrium and its decay rate in the pfcrt-flanking region are consistent with strong directional selective sweeps occurring over only approximately 20-80 sexual generations, especially a single resistant pfcrt haplotype spreading to very high frequencies throughout most of Asia and Africa. The presence of linkage disequilibrium provides a basis for mapping genes under drug selection in P. falciparum.}, added-at = {2010-01-17T03:01:48.000+0100}, author = {Wootton, J C and Feng, X and Ferdig, M T and Cooper, R A and Mu, J and Baruch, D I and Magill, A J and Su, X Z}, biburl = {https://www.bibsonomy.org/bibtex/26bd99e9cfad2c7d3cecae2dabd6ed4ef/gcoop}, description = {Genetic diversity and chloroquine selective sweeps... [Nature. 2002] - PubMed result}, doi = {10.1038/nature00813}, interhash = {487ed7a3a20dc209ec2f2f22f7ebe6bf}, intrahash = {6bd99e9cfad2c7d3cecae2dabd6ed4ef}, journal = {Nature}, keywords = {malaria spatial}, month = Jul, number = 6895, pages = {320-323}, pmid = {12124623}, timestamp = {2010-01-17T03:01:48.000+0100}, title = {Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum}, url = {http://www.ncbi.nlm.nih.gov/pubmed/12124623?dopt=Abstract}, volume = 418, year = 2002 }