%0 Journal Article %1 radmacher1995imaging %A Radmacher, M. %A Fritz, M. %A Hansma, P.K. %D 1995 %J Biophysical Journal %K afm fabrication phd polymer %N 1 %P 264--270 %R 10.1016/S0006-3495(95)79897-6 %T Imaging soft samples with the atomic force microscope: gelatin in water and propanol %U http://www.sciencedirect.com/science/article/pii/S0006349595798976 %V 69 %X We have imaged mica coated with thin gelatin films in water, propanol, and mixtures of these two liquids by atomic force microscopy (AFM). The elastic modulus (Young's modulus) can be tuned from 20 kPa to more than 0.1 \GPa\ depending on the ratio of propanol to water. The resolution is best in pure propanol, on the order of 20 nm, and becomes worse for the softer samples. The degradation in resolution can be understood by considering the elastic indentation of the gelatin caused by the \AFM\ tip. This indentation becomes larger and thus the contact area becomes larger the softer the sample is. Therefore this study may be used to estimate the resolution to be expected with an \AFM\ on other soft samples, such as cells. Nondestructive imaging was possible only by imaging at forces < 1 nN. This was difficult to achieve in contact mode because of drift in the zero load deflection of the cantilever, supposedly caused by temperature drift, but straightforward in tapping mode.

@article{radmacher1995imaging, abstract = {We have imaged mica coated with thin gelatin films in water, propanol, and mixtures of these two liquids by atomic force microscopy (AFM). The elastic modulus (Young's modulus) can be tuned from 20 kPa to more than 0.1 \{GPa\} depending on the ratio of propanol to water. The resolution is best in pure propanol, on the order of 20 nm, and becomes worse for the softer samples. The degradation in resolution can be understood by considering the elastic indentation of the gelatin caused by the \{AFM\} tip. This indentation becomes larger and thus the contact area becomes larger the softer the sample is. Therefore this study may be used to estimate the resolution to be expected with an \{AFM\} on other soft samples, such as cells. Nondestructive imaging was possible only by imaging at forces < 1 nN. This was difficult to achieve in contact mode because of drift in the zero load deflection of the cantilever, supposedly caused by temperature drift, but straightforward in tapping mode. }, added-at = {2013-11-15T14:41:00.000+0100}, author = {Radmacher, M. and Fritz, M. and Hansma, P.K.}, biburl = {https://www.bibsonomy.org/bibtex/230ebda6aa757f725e793b8bcd7ad67eb/bkoch}, description = {Imaging soft samples with the atomic force microscope: gelatin in water and propanol}, doi = {10.1016/S0006-3495(95)79897-6}, interhash = {48f5e03c0a8c30b3452691d633749da6}, intrahash = {30ebda6aa757f725e793b8bcd7ad67eb}, issn = {0006-3495}, journal = {Biophysical Journal }, keywords = {afm fabrication phd polymer}, number = 1, pages = {264--270}, timestamp = {2013-11-15T14:41:00.000+0100}, title = {Imaging soft samples with the atomic force microscope: gelatin in water and propanol }, url = {http://www.sciencedirect.com/science/article/pii/S0006349595798976}, volume = 69, year = 1995 }