%0 Journal Article %1 abdelmohsen2014dereplication %A Abdelmohsen, Usama Ramadan %A Cheng, Cheng %A Viegelmann, Christina %A Zhang, Tong %A Grkovic, Tanja %A Quinn, Ronald J %A Ahmed, Safwat %A Hentschel, Ute %A Edrada-Ebel, RuAngelie %D 2014 %J Mar. Drugs %K myown %N 3 %P 1220-1244 %T Dereplication Strategies for Targeted Isolation of New Anti-trypanosomal Actinosporins A and B from a Marine Sponge Associated-Actinokineospora sp. EG49 %U http://dx.doi.org/10.3390/md12031220 %V 12 %X High resolution Fourier transform mass spectrometry HRFTMS and nuclear magnetic resonance NMR spectroscopy were employed as complementary metabolomic tools to dereplicate the chemical profile of the new and antitrypanosomally active sponge-associated bacterium Actinokineospora sp. EG49 extract. Principal Component PCA, hierarchical clustering HCA, and orthogonal partial least square-discriminant analysis OPLS-DA were used to evaluate the HRFTMS and NMR data of crude extracts from four different fermentation approaches. Statistical analysis identified the best culture one-strain-many-compounds OSMAC condition and extraction procedure, which was used for the isolation of novel bioactive metabolites. As a result, two new O-glycosylated angucyclines, named actinosporins A 1 and B 2, were isolated from the broth culture of Actinokineospora sp. strain EG49, which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structures of actinosporins A and B were determined by 1D- and 2D-NMR techniques, as well as high resolution tandem mass spectrometry. Testing for antiparasitic properties showed that actinosporin A exhibited activity against Trypanosoma brucei brucei with an IC50 value of 15 µM; however no activity was detected against Leishmania major and Plasmodium falciparum, therefore suggesting its selectivity against the parasite Trypanosoma brucei brucei; the causative agent of sleeping sickness.

@article{abdelmohsen2014dereplication, abstract = {High resolution Fourier transform mass spectrometry HRFTMS and nuclear magnetic resonance NMR spectroscopy were employed as complementary metabolomic tools to dereplicate the chemical profile of the new and antitrypanosomally active sponge-associated bacterium Actinokineospora sp. EG49 extract. Principal Component PCA, hierarchical clustering HCA, and orthogonal partial least square-discriminant analysis OPLS-DA were used to evaluate the HRFTMS and NMR data of crude extracts from four different fermentation approaches. Statistical analysis identified the best culture one-strain-many-compounds OSMAC condition and extraction procedure, which was used for the isolation of novel bioactive metabolites. As a result, two new O-glycosylated angucyclines, named actinosporins A 1 and B 2, were isolated from the broth culture of Actinokineospora sp. strain EG49, which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structures of actinosporins A and B were determined by 1D- and 2D-NMR techniques, as well as high resolution tandem mass spectrometry. Testing for antiparasitic properties showed that actinosporin A exhibited activity against Trypanosoma brucei brucei with an IC50 value of 15 µM; however no activity was detected against Leishmania major and Plasmodium falciparum, therefore suggesting its selectivity against the parasite Trypanosoma brucei brucei; the causative agent of sleeping sickness.}, added-at = {2014-04-29T16:01:54.000+0200}, author = {Abdelmohsen, Usama Ramadan and Cheng, Cheng and Viegelmann, Christina and Zhang, Tong and Grkovic, Tanja and Quinn, Ronald J and Ahmed, Safwat and Hentschel, Ute and Edrada-Ebel, RuAngelie}, biburl = {https://www.bibsonomy.org/bibtex/26ec096e81f47ff2fb81098386d37ec4f/cheng1}, interhash = {596cef53587160d26b05002809d1f021}, intrahash = {6ec096e81f47ff2fb81098386d37ec4f}, journal = {Mar. Drugs}, keywords = {myown}, number = 3, pages = {1220-1244}, timestamp = {2014-04-29T16:01:54.000+0200}, title = {Dereplication Strategies for Targeted Isolation of New Anti-trypanosomal Actinosporins A and B from a Marine Sponge Associated-Actinokineospora sp. EG49}, url = {http://dx.doi.org/10.3390/md12031220}, volume = 12, year = 2014 }