%0 Journal Article %1 whiley_new_2004 %A Whiley, D M %A Buda, P J %A Bayliss, J %A Cover, L %A Bates, J %A Sloots, T P %D 2004 %J European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology %K Acid Amplification Bacterial, Chain Female, Gonorrhea, Humans, Male, Neisseria Nucleic Polymerase Pseudogenes, Queensland, Reaction, Sampling Sensitivity Specificity Studies, Techniques, and gonorrhoeae, {DNA,} %N 9 %P 705--710 %R 10.1007/s10096-004-1170-0 %T A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene %U http://www.ncbi.nlm.nih.gov/pubmed/15248092 %V 23 %X The Roche Cobas Amplicor system is widely used for the detection of Neisseria gonorrhoeae but is known to cross react with some commensal Neisseria spp. Therefore, a confirmatory test is required. The most common target for confirmatory tests is the cppB gene of N. gonorrhoeae. However, the cppB gene is also present in other Neisseria spp. and is absent in some N. gonorrhoeae isolates. As a result, laboratories targeting this gene run the risk of obtaining both false-positive and false-negative results. In the study presented here, a newly developed N. gonorrhoeae LightCycler assay (NGpapLC) targeting the N. gonorrhoeae porA pseudogene was tested. The NGpapLC assay was used to test 282 clinical samples, and the results were compared to those obtained using a testing algorithm combining the Cobas Amplicor System (Roche Diagnostics, Sydney, Australia) and an in-house LightCycler assay targeting the cppB gene (cppB-LC). In addition, the specificity of the NGpapLC assay was investigated by testing a broad panel of bacteria including isolates of several Neisseria spp. The NGpapLC assay proved to have comparable clinical sensitivity to the cppB-LC assay. In addition, testing of the bacterial panel showed the NGpapLC assay to be highly specific for N. gonorrhoeae DNA. The results of this study show the NGpapLC assay is a suitable alternative to the cppB-LC assay for confirmation of N. gonorrhoeae-positive results obtained with Cobas Amplicor.

@article{whiley_new_2004, abstract = {The Roche Cobas Amplicor system is widely used for the detection of Neisseria gonorrhoeae but is known to cross react with some commensal Neisseria spp. Therefore, a confirmatory test is required. The most common target for confirmatory tests is the {cppB} gene of N. gonorrhoeae. However, the {cppB} gene is also present in other Neisseria spp. and is absent in some N. gonorrhoeae isolates. As a result, laboratories targeting this gene run the risk of obtaining both false-positive and false-negative results. In the study presented here, a newly developed N. gonorrhoeae {LightCycler} assay {(NGpapLC)} targeting the N. gonorrhoeae {porA} pseudogene was tested. The {NGpapLC} assay was used to test 282 clinical samples, and the results were compared to those obtained using a testing algorithm combining the Cobas Amplicor System {(Roche} Diagnostics, Sydney, Australia) and an in-house {LightCycler} assay targeting the {cppB} gene {(cppB-LC).} In addition, the specificity of the {NGpapLC} assay was investigated by testing a broad panel of bacteria including isolates of several Neisseria spp. The {NGpapLC} assay proved to have comparable clinical sensitivity to the {cppB-LC} assay. In addition, testing of the bacterial panel showed the {NGpapLC} assay to be highly specific for N. gonorrhoeae {DNA.} The results of this study show the {NGpapLC} assay is a suitable alternative to the {cppB-LC} assay for confirmation of N. gonorrhoeae-positive results obtained with Cobas Amplicor.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Whiley, D M and Buda, P J and Bayliss, J and Cover, L and Bates, J and Sloots, T P}, biburl = {https://www.bibsonomy.org/bibtex/20412895bb39437bc8bdd97eac3671ef1/jelias}, doi = {10.1007/s10096-004-1170-0}, interhash = {5a2178c3c4d08c9259eb03f56d4e5e76}, intrahash = {0412895bb39437bc8bdd97eac3671ef1}, issn = {0934-9723}, journal = {European Journal of Clinical Microbiology \& Infectious Diseases: Official Publication of the European Society of Clinical Microbiology}, keywords = {Acid Amplification Bacterial, Chain Female, Gonorrhea, Humans, Male, Neisseria Nucleic Polymerase Pseudogenes, Queensland, Reaction, Sampling Sensitivity Specificity Studies, Techniques, and gonorrhoeae, {DNA,}}, month = sep, note = {{PMID:} 15248092}, number = 9, pages = {705--710}, timestamp = {2011-03-11T10:05:39.000+0100}, title = {A new confirmatory Neisseria gonorrhoeae real-time {PCR} assay targeting the {porA} pseudogene}, url = {http://www.ncbi.nlm.nih.gov/pubmed/15248092}, volume = 23, year = 2004 }