%0 Journal Article %1 Moore2014Transcription %A Moore, Richard %A Chandrahas, Anita %A Bleris, Leonidas %D 2014 %J ACS synthetic biology %K synthetic-biology transcription %T Transcription Activator-like Effectors: A Toolkit for Synthetic Biology. %U http://view.ncbi.nlm.nih.gov/pubmed/24933470 %X Transcription activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. TALEs assist infections by binding to specific DNA sequences and activating the expression of host genes. Recent results show that TALE proteins consist of a central repeat domain, which determines the DNA targeting specificity and can be rapidly synthesized de novo. Considering the highly modular nature of TALEs, their versatility, and the ease of constructing these proteins, this technology can have important implications for synthetic biology applications. Here, we review developments in the area with a particular focus on modifications for custom and controllable gene regulation.

@article{Moore2014Transcription, abstract = {Transcription activator-like effectors ({TALEs}) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. {TALEs} assist infections by binding to specific {DNA} sequences and activating the expression of host genes. Recent results show that {TALE} proteins consist of a central repeat domain, which determines the {DNA} targeting specificity and can be rapidly synthesized de novo. Considering the highly modular nature of {TALEs}, their versatility, and the ease of constructing these proteins, this technology can have important implications for synthetic biology applications. Here, we review developments in the area with a particular focus on modifications for custom and controllable gene regulation.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Moore, Richard and Chandrahas, Anita and Bleris, Leonidas}, biburl = {https://www.bibsonomy.org/bibtex/26cfdb05102756ac563f9d81a6680c6e8/karthikraman}, citeulike-article-id = {13239583}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/24933470}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=24933470}, day = 13, interhash = {5f9bdc4e2b24357aaa1e9c20b746ea37}, intrahash = {6cfdb05102756ac563f9d81a6680c6e8}, issn = {2161-5063}, journal = {ACS synthetic biology}, keywords = {synthetic-biology transcription}, month = feb, pmid = {24933470}, posted-at = {2014-06-23 05:27:03}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Transcription Activator-like Effectors: A Toolkit for Synthetic Biology.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/24933470}, year = 2014 }