%0 Journal Article %1 citeulike:566865 %A Smith, S. J. %A Henderson, L. O. %A Hannon, W. H. %A Cooper, G. R. %C Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333. %D 1990 %J Clin Chem %K methodology immunoassay apob %N 2 %P 290--296 %T Effects of analytical method and lyophilized sera on measurements of apolipoproteins A-I and B: an international survey. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2105859 %V 36 %X In 1987 a collaborative study was initiated with 140 laboratories worldwide to evaluate the effects of analytical method and lyophilization on the measurement of different concentrations of apolipoproteins (apo) A-I and B in four lyophilized serum pool samples. This survey confirmed that the lyophilized apo Reference Material of the International Union of Immunological Societies (IUIS) is useful for apo A-I assays as an international serum-based reference material, because among-method variation is negligible. The apo A-I concentration value of 1.24 g/L is now assigned to the IUIS Reference Material (CDC 1883) by a Centers for Disease Control RIA in-house reference method. Use of lyophilized serum preparation as a reference material for some modes of apo B measurement is questionable because of lyophilization and matrix effects. Both radial immunodiffusion and liquid immunoprecipitin methods demonstrated bias in measured apo B concentrations, compared with overall method-weighted means values on the IUIS Reference Material. Because of the uncertainty associated with LDL primary standard, protein analysis, and concentration differences among analytical methods, assigning a single apo B concentration value to the IUIS Reference Material appears inadvisable at present.

@article{citeulike:566865, abstract = {In 1987 a collaborative study was initiated with 140 laboratories worldwide to evaluate the effects of analytical method and lyophilization on the measurement of different concentrations of apolipoproteins (apo) A-I and B in four lyophilized serum pool samples. This survey confirmed that the lyophilized apo Reference Material of the International Union of Immunological Societies (IUIS) is useful for apo A-I assays as an international serum-based reference material, because among-method variation is negligible. The apo A-I concentration value of 1.24 g/L is now assigned to the IUIS Reference Material (CDC 1883) by a Centers for Disease Control RIA in-house reference method. Use of lyophilized serum preparation as a reference material for some modes of apo B measurement is questionable because of lyophilization and matrix effects. Both radial immunodiffusion and liquid immunoprecipitin methods demonstrated bias in measured apo B concentrations, compared with overall method-weighted means values on the IUIS Reference Material. Because of the uncertainty associated with LDL primary standard, protein analysis, and concentration differences among analytical methods, assigning a single apo B concentration value to the IUIS Reference Material appears inadvisable at present.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.}, author = {Smith, S. J. and Henderson, L. O. and Hannon, W. H. and Cooper, G. R.}, biburl = {https://www.bibsonomy.org/bibtex/2e29d89409fc67194beed3d2ce661395e/biblio24}, citeulike-article-id = {566865}, interhash = {6086639a8011197f2ea1371da16eddfa}, intrahash = {e29d89409fc67194beed3d2ce661395e}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {methodology immunoassay apob}, month = {February}, number = 2, pages = {290--296}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Effects of analytical method and lyophilized sera on measurements of apolipoproteins A-I and B: an international survey.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=2105859}, volume = 36, year = 1990 }