%0 Journal Article %1 noauthororeditor %A Ganesan, Sridharan %A Luu, Thuy Thanh %A Chambers, BJ %A Meinke, Stephan %A Brodin, Petter %A Vivier, Eric %A Wetzel, Dawn M %A Koleske, Anthony J %A Kadri, Nadir %A Höglund, Petter %D 2017 %J Scandinavian journal of immunology %K NK %N 3 %P 135-142 %R 10.1111/sji.12574 %T The Abl-1 Kinase is Dispensable for NK Cell Inhibitory Signalling and is not Involved in Murine NK Cell Education. %V 86 %X Natural killer (NK) cell responsiveness in the mouse is determined in an education process guided by inhibitory Ly49 and NKG2A receptors binding to MHC class I molecules. It has been proposed that inhibitory signalling in human NK cells involves Abl-1 (c-Abl)-mediated phosphorylation of Crk, lowering NK cell function via disruption of a signalling complex including C3G and c-Cbl, suggesting that NK cell education might involve c-Abl. Mice deficient in c-Abl expression specifically in murine NK cells displayed normal inhibitory and activating receptor repertoires. Furthermore, c-Abl-deficient NK cells fluxed Ca2+ normally after triggering of ITAM receptors, killed YAC-1 tumour cells efficiently and showed normal, or even slightly elevated, capacity to produce IFN-γ after activating receptor stimulation. Consistent with these results, c-Abl deficiency in NK cells did not affect NK cell inhibition via the receptors Ly49G2, Ly49A and NKG2A. We conclude that signalling downstream of murine inhibitory receptors does not involve c-Abl and that c-Abl plays no major role in NK cell education in the mouse.

@article{noauthororeditor, abstract = {Natural killer (NK) cell responsiveness in the mouse is determined in an education process guided by inhibitory Ly49 and NKG2A receptors binding to MHC class I molecules. It has been proposed that inhibitory signalling in human NK cells involves Abl-1 (c-Abl)-mediated phosphorylation of Crk, lowering NK cell function via disruption of a signalling complex including C3G and c-Cbl, suggesting that NK cell education might involve c-Abl. Mice deficient in c-Abl expression specifically in murine NK cells displayed normal inhibitory and activating receptor repertoires. Furthermore, c-Abl-deficient NK cells fluxed Ca2+ normally after triggering of ITAM receptors, killed YAC-1 tumour cells efficiently and showed normal, or even slightly elevated, capacity to produce IFN-γ after activating receptor stimulation. Consistent with these results, c-Abl deficiency in NK cells did not affect NK cell inhibition via the receptors Ly49G2, Ly49A and NKG2A. We conclude that signalling downstream of murine inhibitory receptors does not involve c-Abl and that c-Abl plays no major role in NK cell education in the mouse.}, added-at = {2020-04-02T19:22:48.000+0200}, author = {Ganesan, Sridharan and Luu, Thuy Thanh and Chambers, BJ and Meinke, Stephan and Brodin, Petter and Vivier, Eric and Wetzel, Dawn M and Koleske, Anthony J and Kadri, Nadir and Höglund, Petter}, biburl = {https://www.bibsonomy.org/bibtex/2a4c5204c78dfd38a1083c20a25b7589d/lkanth}, description = {The Abl‐1 Kinase is Dispensable for NK Cell Inhibitory Signalling and is not Involved in Murine NK Cell Education - Ganesan - 2017 - Scandinavian Journal of Immunology - Wiley Online Library}, doi = {10.1111/sji.12574}, interhash = {64e1e8afaaf875ffc2755eacb791c40e}, intrahash = {a4c5204c78dfd38a1083c20a25b7589d}, issn = {0300-9475}, journal = {Scandinavian journal of immunology}, keywords = {NK}, month = {September}, number = 3, pages = {135-142}, timestamp = {2020-04-02T19:22:48.000+0200}, title = {The Abl-1 Kinase is Dispensable for NK Cell Inhibitory Signalling and is not Involved in Murine NK Cell Education.}, volume = 86, year = 2017 }