%0 Journal Article %1 trabesinger2002continuous %A Trabesinger, Werner %A Hübner, Christian G. %A Hecht, Bert %A Wild, Urs P. %D 2002 %J Rev. Sci. Instrum. %K experiment lifetime-measurements nano-optics single-molecules %N 8 %P 3122-3124 %R 10.1063/1.1488676 %T Continuous real-time measurement of fluorescence lifetimes %V 73 %X We report on the continuous measurement of fluorescence lifetimes at low light levels. Fluorescence photons following pulsed excitation generate a pulse sequence with exponentially distributed amplitudes and interphoton times at the output of a time-to-amplitude converter. This sequence is turned into a continuous step function and is time averaged with an adjustable bandwidth. For a single-exponential decay, our approach yields identical results as would be obtained from fitting fluorescence decays, while being a real-time technique. The proposed technique performs especially well at low count rates. We demonstrate the applicability of the method at the example of confocal fluorescence lifetime imaging of single molecules.

@article{trabesinger2002continuous, abstract = {We report on the continuous measurement of fluorescence lifetimes at low light levels. Fluorescence photons following pulsed excitation generate a pulse sequence with exponentially distributed amplitudes and interphoton times at the output of a time-to-amplitude converter. This sequence is turned into a continuous step function and is time averaged with an adjustable bandwidth. For a single-exponential decay, our approach yields identical results as would be obtained from fitting fluorescence decays, while being a real-time technique. The proposed technique performs especially well at low count rates. We demonstrate the applicability of the method at the example of confocal fluorescence lifetime imaging of single molecules.}, added-at = {2020-02-17T11:32:38.000+0100}, author = {Trabesinger, Werner and Hübner, Christian G. and Hecht, Bert and Wild, Urs P.}, biburl = {https://www.bibsonomy.org/bibtex/2a0a5a42b92fefe4c50064b93f7d459f3/ep5optics}, day = 25, doi = {10.1063/1.1488676}, file = {Snapshot:C\:\\Users\\scherzad\\Zotero\\storage\\C9VVT3R3\\1.html:text/html;Trabesinger et al. - 2002 - Continuous real-time measurement of fluorescence l.pdf:C\:\\Users\\scherzad\\Zotero\\storage\\FU5ZST4Y\\Trabesinger et al. - 2002 - Continuous real-time measurement of fluorescence l.pdf:application/pdf}, interhash = {6e2c9ec0fc9194c7ae18a97a5eadb321}, intrahash = {a0a5a42b92fefe4c50064b93f7d459f3}, issn = {0034-6748}, journal = {Rev. Sci. Instrum.}, keywords = {experiment lifetime-measurements nano-optics single-molecules}, month = {07}, number = 8, pages = {3122-3124}, timestamp = {2020-02-17T11:32:38.000+0100}, title = {Continuous real-time measurement of fluorescence lifetimes}, urldate = {2020-02-17}, volume = 73, year = 2002 }