%0 Journal Article %1 Schwaenen.2009 %A Schwaenen, Carsten %A Viardot, Andreas %A Berger, Hilmar %A Barth, Thomas F E, %A Bentink, Stefan %A Döhner, Hartmut %A Enz, Martina %A Feller, Alfred C. %A Hansmann, Martin-Leo %A Hummel, Michael %A Kestler, Hans A. %A Klapper, Wolfram %A Kreuz, Markus %A Lenze, Dido %A Loeffler, Markus %A Möller, Peter %A Müller-Hermelink, Hans-Konrad %A Ott, German %A Rosolowski, Maciej %A Rosenwald, Andreas %A Ruf, Sandra %A Siebert, Reiner %A Spang, Rainer %A Stein, Harald %A Truemper, Lorenz %A Lichter, Peter %A Bentz, Martin %A Wessendorf, Swen %D 2009 %J Genes, chromosomes & cancer %K Adolescent Adult Aged Aged,_80_and_over Analysis_of_Variance Child Chromosome_Aberrations Comparative_Genomic_Hybridization Female Humans In_Situ_Hybridization,_Fluorescence Kaplan-Meier_Estimate Lymphoma,_Follicular/genetics/metabolism/mortality/pathology Male Middle_Aged Oligonucleotide_Array_Sequence_Analysis/methods Young_Adult %N 1 %P 39–54 %T Microarray-based genomic profiling reveals novel genomic aberrations in follicular lymphoma which associate with patient survival and gene expression status %V 48 %X Follicular lymphoma (FL) is characterized by a large number of chromosomal aberrations. However, their exact genomic extension and involved target genes remain to be determined. For this purpose, we used array-based intermediate-high resolution genomic profiling in combination with Affymetrix gene expression analysis. Tumor specimens from 128 FL patients were analyzed for the presence of genomic aberrations and the results were correlated to clinical data sets and mRNA expression levels. In 114 (89\%) of the 128 analyzed cases, a total of 688 genomic aberrations (384 gains/amplifications and 304 losses) were detected. Frequent genomic aberrations were: -1p36 (18\%), +2p15 (24\%), -3q (14\%), -6q (25\%), +7p (19\%), +7q (23\%), +8q (14\%), -9p (16\%), -11q (15\%), +12q (20\%), -13q (11\%), -17p (16\%), +18p (18\%), and +18q (28\%). Critical segments of these imbalances were delineated to genomic fragments with a minimum size down to 0.2 Mb. By comparison of these with mRNA gene expression data, putative candidate genes were identified. Moreover, we found that deletions affecting the tumor suppressor gene CDKN2A/B on 9p21 were detected in nontransformed FL grade I-II. For this aberration as well as for -6q25 and -6q26, an association with inferior survival was observed.

@article{Schwaenen.2009, abstract = {Follicular lymphoma (FL) is characterized by a large number of chromosomal aberrations. However, their exact genomic extension and involved target genes remain to be determined. For this purpose, we used array-based intermediate-high resolution genomic profiling in combination with Affymetrix gene expression analysis. Tumor specimens from 128 FL patients were analyzed for the presence of genomic aberrations and the results were correlated to clinical data sets and mRNA expression levels. In 114 (89\%) of the 128 analyzed cases, a total of 688 genomic aberrations (384 gains/amplifications and 304 losses) were detected. Frequent genomic aberrations were: -1p36 (18\%), +2p15 (24\%), -3q (14\%), -6q (25\%), +7p (19\%), +7q (23\%), +8q (14\%), -9p (16\%), -11q (15\%), +12q (20\%), -13q (11\%), -17p (16\%), +18p (18\%), and +18q (28\%). Critical segments of these imbalances were delineated to genomic fragments with a minimum size down to 0.2 Mb. By comparison of these with mRNA gene expression data, putative candidate genes were identified. Moreover, we found that deletions affecting the tumor suppressor gene CDKN2A/B on 9p21 were detected in nontransformed FL grade I-II. For this aberration as well as for -6q25 and -6q26, an association with inferior survival was observed.}, added-at = {2014-10-15T15:04:26.000+0200}, author = {Schwaenen, Carsten and Viardot, Andreas and Berger, Hilmar and {Barth, Thomas F E} and Bentink, Stefan and Döhner, Hartmut and Enz, Martina and Feller, Alfred C. and Hansmann, Martin-Leo and Hummel, Michael and Kestler, Hans A. and Klapper, Wolfram and Kreuz, Markus and Lenze, Dido and Loeffler, Markus and Möller, Peter and Müller-Hermelink, Hans-Konrad and Ott, German and Rosolowski, Maciej and Rosenwald, Andreas and Ruf, Sandra and Siebert, Reiner and Spang, Rainer and Stein, Harald and Truemper, Lorenz and Lichter, Peter and Bentz, Martin and Wessendorf, Swen}, biburl = {https://www.bibsonomy.org/bibtex/26ff78b818969e14973619f6bf7de77ad/drtester}, interhash = {8a1451f6058b468a6626adf3105f9514}, intrahash = {6ff78b818969e14973619f6bf7de77ad}, journal = {Genes, chromosomes \& cancer}, keywords = {Adolescent Adult Aged Aged,_80_and_over Analysis_of_Variance Child Chromosome_Aberrations Comparative_Genomic_Hybridization Female Humans In_Situ_Hybridization,_Fluorescence Kaplan-Meier_Estimate Lymphoma,_Follicular/genetics/metabolism/mortality/pathology Male Middle_Aged Oligonucleotide_Array_Sequence_Analysis/methods Young_Adult}, number = 1, pages = {39–54}, timestamp = {2014-10-15T15:04:26.000+0200}, title = {Microarray-based genomic profiling reveals novel genomic aberrations in follicular lymphoma which associate with patient survival and gene expression status}, volume = 48, year = 2009 }