Article,

Superaufgelöste Mikroskopie: Pilze unter Beobachtung

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BIOspektrum, 27 (4): 380--382 (2021)
DOI: 10.1007/s12268-021-1592-6

Abstract

The diffraction limit of light confines fluorescence imaging of subcellular structures in fungi. Different super-resolution methods are available for the analysis of fungi that we briefly discuss. We exploit the filamentous fungus Fusarium fujikuroi expressing a YFP-labeled membrane protein showing the benefit of correlative light- and electron microscopy (CLEM), that combines structured illumination microscopy (SIM) and scanning election microscopy (SEM).

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