Аннотация
Although human tumor-derived cell lines play an important role in
the investigation of cancer biology and genetics, there is no comprehensive
study comparing tumor cell line properties with those of the individual
tumors from which they were derived. We compared the properties of
a series of 18 human breast cancer cell lines that were cultured
for a median period of 25 months (range, 9-60 months) and their corresponding
archival tumor tissues. We compared morphological characteristics,
ploidy, and immunohistochemical expression of estrogen receptors,
progesterone receptors, and HER2/neu and p53 proteins. For 17 of
these cases, we also tested for allelic losses at 18 chromosomal
regions frequently deleted in breast tumors using 51 polymorphic
microsatellite markers, and we determined the TP53 gene mutation
status in exons 5 to 10. There was an excellent correlation between
the breast tumor cell lines and their corresponding tumor tissues
for morphological features (100%); presence of aneuploidy (87%);
immunohistochemical expression of estrogen receptors (87%), progesterone
receptors (73%), and HER2/neu (93%) and p53 proteins (100%); allelic
loss at all of the chromosomal regions analyzed (82-100% concordance);
and TP53 gene mutations (75%). The same parental allele was lost
in 279 (99%) of 281 of the comparisons of allelic losses. The fractional
allelic loss indices (a reflection of the total allelic loss) of
the cell lines and their corresponding tumor tissues were identical
or similar in 15 (88%) of 17 paired comparisons. Although our previous
studies (A. Gazdar et al., Int. J. Cancer, in press) indicated that
only a subset of primary breast carcinomas that have several features
indicative of advanced tumors with poor prognosis can be successfully
cultured, the cell lines retain the properties of their parental
tumors for lengthy culture periods and, thus, provide suitable model
systems for biomedical studies.
- adult
- aged
- aged,_80_and_over
- breast_neoplasms,_genetics/pathology
- dna,_neoplasm,_analysis
- dna_mutational_analysis
- humans
- immunohistochemistry
- loss_of_heterozygosity
- middle_aged
- ploidies
- receptor,_erbb-2,_biosynthesis
- receptors,_estrogen,_biosynthesis
- receptors,_progesterone,_biosynthesis
- time_factors
- tumor_cells,_cultured
- tumor_suppressor_protein_p53,_genetics
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