Abstract
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100\% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
- a,
- analysis,
- aureus
- bacterial
- bacterial,
- humans,
- infections,
- laboratories,
- observer
- of
- protein
- reproducibility
- results,
- sequence
- software,
- staphylococcal
- staphylococcus
- techniques,
- typing
- variation,
- {dna},
Users
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