%0 Journal Article %1 Saft_2001_2727 %A Saftenku, E. %A Williams, A. J. %A Sitsapesan, R. %D 2001 %J Biophys. J. %K 11371448 Animals, Calcium Calcium, Chains, Channel Channel, Functions, Gating, Gov't, Ion Kinetics, Likelihood Markov Myocardium, Non-U.S. Patch-Clamp Receptor Release Research Ryanodine Sheep, Support, Techniques, Transport, %N 6 %P 2727--2741 %T Markovian models of low and high activity levels of cardiac ryanodine receptors. %U http://www.biophysj.org/cgi/content/full/80/6/2727 %V 80 %X The modal gating behavior of single sheep cardiac sarcoplasmic reticulum (SR) Ca$^2+$-release/ryanodine receptor (RyR) channels was assessed. We find that the gating of RyR channels spontaneously shifts between high (H) and low (L) levels of activity and inactive periods where no channel openings are detected (I). Moreover, we find that there is evidence for multiple gating modes within H activity, which we term H1 and H2 mode. Our results demonstrate that the underlying mechanisms regulating gating are similar in native and purified channels. Dwell-time distributions of L activity were best fitted by three open and five closed significant exponential components whereas dwell-time distributions of H1 activity were best fitted by two to three open and four closed significant exponential components. Increases in cytosolic Ca$^2+$ cause an increase in open probability (Po) within L activity and an increase in the probability of occurrence of H activity. Open lifetime distributions within L activity were Ca$^2+$ independent whereas open lifetime distributions within H activity were Ca$^2+$ dependent. This study is the first attempt to estimate RyR single-channel kinetic parameters from sequences of idealized dwell-times and to develop kinetic models of RyR gating using the criterion of maximum likelihood. We propose distinct kinetic schemes for L, H1, and H2 activity that describe the major features of sheep cardiac RyR channel gating at these levels of activity.

@article{Saft_2001_2727, abstract = {The modal gating behavior of single sheep cardiac sarcoplasmic reticulum (SR) {C}a$^{2+}$-release/ryanodine receptor (RyR) channels was assessed. We find that the gating of RyR channels spontaneously shifts between high (H) and low (L) levels of activity and inactive periods where no channel openings are detected (I). Moreover, we find that there is evidence for multiple gating modes within H activity, which we term H1 and H2 mode. Our results demonstrate that the underlying mechanisms regulating gating are similar in native and purified channels. Dwell-time distributions of L activity were best fitted by three open and five closed significant exponential components whereas dwell-time distributions of H1 activity were best fitted by two to three open and four closed significant exponential components. Increases in cytosolic [{C}a$^{2+}$] cause an increase in open probability (Po) within L activity and an increase in the probability of occurrence of H activity. Open lifetime distributions within L activity were {C}a$^{2+}$ independent whereas open lifetime distributions within H activity were {C}a$^{2+}$ dependent. This study is the first attempt to estimate RyR single-channel kinetic parameters from sequences of idealized dwell-times and to develop kinetic models of RyR gating using the criterion of maximum likelihood. We propose distinct kinetic schemes for L, H1, and H2 activity that describe the major features of sheep cardiac RyR channel gating at these levels of activity.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Saftenku, E. and Williams, A. J. and Sitsapesan, R.}, biburl = {https://www.bibsonomy.org/bibtex/27a11079197f2ca9cbdd7db96ce7b0df8/hake}, description = {The whole bibliography file I use.}, file = {Saft_2001_2727.pdf:Saft_2001_2727.pdf:PDF}, interhash = {90fc463fd7188dd702c19dbfc3e5a717}, intrahash = {7a11079197f2ca9cbdd7db96ce7b0df8}, journal = {Biophys. J.}, keywords = {11371448 Animals, Calcium Calcium, Chains, Channel Channel, Functions, Gating, Gov't, Ion Kinetics, Likelihood Markov Myocardium, Non-U.S. Patch-Clamp Receptor Release Research Ryanodine Sheep, Support, Techniques, Transport,}, month = Jun, number = 6, pages = {2727--2741}, pmid = {11371448}, timestamp = {2009-06-03T11:21:28.000+0200}, title = {Markovian models of low and high activity levels of cardiac ryanodine receptors.}, url = {http://www.biophysj.org/cgi/content/full/80/6/2727}, volume = 80, year = 2001 }