%0 Journal Article %1 huang2023cipk23 %A Huang, S. %A Maierhofer, T. %A Hashimoto, K. %A Xu, X. %A Karimi, S. M. %A Muller, H. %A Geringer, M. A. %A Wang, Y. %A Kudla, J. %A De Smet, I. %A Hedrich, R. %A Geiger, D. %A Roelfsema, M. R. G. %D 2023 %J New Phytol %K Abscisic Acid/metabolism myOwn %N 1 %P 270-282 %R 10.1111/nph.18708 %T The CIPK23 protein kinase represses SLAC1-type anion channels in Arabidopsis guard cells and stimulates stomatal opening %U https://www.ncbi.nlm.nih.gov/pubmed/36597715 %V 238 %X Guard cells control the opening of stomatal pores in the leaf surface, with the use of a network of protein kinases and phosphatases. Loss of function of the CBL-interacting protein kinase 23 (CIPK23) was previously shown to decrease the stomatal conductance, but the molecular mechanisms underlying this response still need to be clarified. CIPK23 was specifically expressed in Arabidopsis guard cells, using an estrogen-inducible system. Stomatal movements were linked to changes in ion channel activity, determined with double-barreled intracellular electrodes in guard cells and with the two-electrode voltage clamp technique in Xenopus oocytes. Expression of the phosphomimetic variant CIPK23(T190D) enhanced stomatal opening, while the natural CIPK23 and a kinase-inactive CIPK23(K60N) variant did not affect stomatal movements. Overexpression of CIPK23(T190D) repressed the activity of S-type anion channels, while their steady-state activity was unchanged by CIPK23 and CIPK23(K60N) . We suggest that CIPK23 enhances the stomatal conductance at favorable growth conditions, via the regulation of several ion transport proteins in guard cells. The inhibition of SLAC1-type anion channels is an important facet of this response.

@article{huang2023cipk23, abstract = {Guard cells control the opening of stomatal pores in the leaf surface, with the use of a network of protein kinases and phosphatases. Loss of function of the CBL-interacting protein kinase 23 (CIPK23) was previously shown to decrease the stomatal conductance, but the molecular mechanisms underlying this response still need to be clarified. CIPK23 was specifically expressed in Arabidopsis guard cells, using an estrogen-inducible system. Stomatal movements were linked to changes in ion channel activity, determined with double-barreled intracellular electrodes in guard cells and with the two-electrode voltage clamp technique in Xenopus oocytes. Expression of the phosphomimetic variant CIPK23(T190D) enhanced stomatal opening, while the natural CIPK23 and a kinase-inactive CIPK23(K60N) variant did not affect stomatal movements. Overexpression of CIPK23(T190D) repressed the activity of S-type anion channels, while their steady-state activity was unchanged by CIPK23 and CIPK23(K60N) . We suggest that CIPK23 enhances the stomatal conductance at favorable growth conditions, via the regulation of several ion transport proteins in guard cells. The inhibition of SLAC1-type anion channels is an important facet of this response.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Huang, S. and Maierhofer, T. and Hashimoto, K. and Xu, X. and Karimi, S. M. and Muller, H. and Geringer, M. A. and Wang, Y. and Kudla, J. and De Smet, I. and Hedrich, R. and Geiger, D. and Roelfsema, M. R. G.}, biburl = {https://www.bibsonomy.org/bibtex/230f2e7be7e244771f7e6a3c74705f8ba/jvsi_all}, doi = {10.1111/nph.18708}, interhash = {9ecb320cf397e660f073443e0e9e4f3e}, intrahash = {30f2e7be7e244771f7e6a3c74705f8ba}, issn = {1469-8137 (Electronic) 0028-646X (Linking)}, journal = {New Phytol}, keywords = {Abscisic Acid/metabolism myOwn}, note = {Huang, Shouguang Maierhofer, Tobias Hashimoto, Kenji Xu, Xiangyu Karimi, Sohail M Muller, Heike Geringer, Michael A Wang, Yi Kudla, Jorg De Smet, Ive Hedrich, Rainer Geiger, Dietmar Roelfsema, M Rob G eng Research Support, Non-U.S. Gov't England 2023/01/05 New Phytol. 2023 Apr;238(1):270-282. doi: 10.1111/nph.18708. Epub 2023 Jan 27.}, number = 1, pages = {270-282}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {The CIPK23 protein kinase represses SLAC1-type anion channels in Arabidopsis guard cells and stimulates stomatal opening}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/36597715}, volume = 238, year = 2023 }