%0 Journal Article %1 abdullahi_abdulkakdir_2021_4644081 %A Abdulkakdir, Abdullahi %A Dania, Omeremime Elizabeth %A Mohammed, Bala Alkali %A Mohammed, Yahaya Abubakar %A Umar, Maimuna Bello %A Garba, Rahinat %D 2021 %J GSC Advanced Research and Reviews %K Activity %N 3 %P 053-060 %R 10.30574/gscarr.2021.6.3.0032 %T Effect of Vernonia amygdalina on phospholipase activity from Naja Mossambica (Cobra) %U https://gsconlinepress.com/journals/gscarr/content/effect-vernonia-amygdalina-phospholipase-activity-naja-mossambica-cobra %V 6 %X Background: Phospholipases are one of the numerous enzymes found in the Naja mossambica venom. They play a major role in snakebite envenomation, and also responsible for the hydrolysis of a phospholipid, disrupting the membrane integrity. In this study, we evaluated the effect of Vernonia amygdalina on Phospholipase activity from Naja mossambica (Cobra) Results: Partially purified phospholipase had maximal velocity (Vmax) and Michaelis Menten constant (Km) of 7.6 × 10-5 mol/min and 1.7mg/ml, while the crude phospholipase had Vmax and Km of 9.4 × 10-5mol/min and 2.5mg/ml respectively. Inhibition study of aqueous extracts of Vernonia amygdalina leaf shows that the extract is a potent inhibitor of crude phospholipase in a dose-dependent pattern. The different doses of extract 15 %, 10 % and 5% produced percentage inhibition of 74.04 %, 78.6 % and 86.63% respectively. The kinetic binding constant (Ki) values of crude phospholipase for different concentrations of extracts 5%, 10% and 15% were 0.21mg/ml, 0.29mg/ml and 0.39mg/ml, while the partially purified phospholipase for different concentrations of extracts 5%, 10% and 15% were were 0.48mg/ml, 0.42mg/ml and 0.41mg/ml respectively. It can be deduced from the results that the extract inhibits the phospholipase activity in an uncompetitive manner. Conclusion: Aqueous extract of Vernonia amygdalina leaves may contain some bioactive agents that could serve as potent inhibitors of phospholipase from Naja mossambica venom.

@article{abdullahi_abdulkakdir_2021_4644081, abstract = {Background: Phospholipases are one of the numerous enzymes found in the Naja mossambica venom. They play a major role in snakebite envenomation, and also responsible for the hydrolysis of a phospholipid, disrupting the membrane integrity. In this study, we evaluated the effect of Vernonia amygdalina on Phospholipase activity from Naja mossambica (Cobra) Results: Partially purified phospholipase had maximal velocity (Vmax) and Michaelis Menten constant (Km) of 7.6 × 10-5 mol/min and 1.7mg/ml, while the crude phospholipase had Vmax and Km of 9.4 × 10-5mol/min and 2.5mg/ml respectively. Inhibition study of aqueous extracts of Vernonia amygdalina leaf shows that the extract is a potent inhibitor of crude phospholipase in a dose-dependent pattern. The different doses of extract 15 %, 10 % and 5% produced percentage inhibition of 74.04 %, 78.6 % and 86.63% respectively. The kinetic binding constant (Ki) values of crude phospholipase for different concentrations of extracts 5%, 10% and 15% were 0.21mg/ml, 0.29mg/ml and 0.39mg/ml, while the partially purified phospholipase for different concentrations of extracts 5%, 10% and 15% were were 0.48mg/ml, 0.42mg/ml and 0.41mg/ml respectively. It can be deduced from the results that the extract inhibits the phospholipase activity in an uncompetitive manner. Conclusion: Aqueous extract of Vernonia amygdalina leaves may contain some bioactive agents that could serve as potent inhibitors of phospholipase from Naja mossambica venom.}, added-at = {2021-03-31T13:17:34.000+0200}, author = {Abdulkakdir, Abdullahi and Dania, Omeremime Elizabeth and Mohammed, Bala Alkali and Mohammed, Yahaya Abubakar and Umar, Maimuna Bello and Garba, Rahinat}, biburl = {https://www.bibsonomy.org/bibtex/238f196eb58e2feabdb841432fbe67a5c/gscarrjournal}, doi = {10.30574/gscarr.2021.6.3.0032}, interhash = {1a720af111c38fcc978ded0583146e19}, intrahash = {38f196eb58e2feabdb841432fbe67a5c}, issn = {2582-4597}, journal = {GSC Advanced Research and Reviews}, keywords = {Activity}, month = mar, number = 3, pages = {053-060}, timestamp = {2021-03-31T13:17:34.000+0200}, title = {Effect of Vernonia amygdalina on phospholipase activity from Naja Mossambica (Cobra)}, url = {https://gsconlinepress.com/journals/gscarr/content/effect-vernonia-amygdalina-phospholipase-activity-naja-mossambica-cobra}, volume = 6, year = 2021 }