Abstract
During storage, red blood cells (RBCs) for transfusion purposes suffer
progressive deterioration. Sialylated glycoproteins of the RBC membrane
are responsible for a negatively charged surface which creates a
repulsive electrical zeta potential. These charges help prevent the
interaction between RBCs and other cells, and especially among each
RBCs. Reports in the literature have stated that RBCs sialylated
glycoproteins can be sensitive to enzymes released by leukocyte
degranulation. Thus, the aim of this study was, by using an optical
tweezers as a biomedical tool, to measure the zeta potential in standard
RBCs units and in leukocyte reduced RBC units (collected in CPD-SAGM)
during storage. Optical tweezers is a sensitive tool that uses light for
measuring cell biophysical properties which are important for clinical
and research purposes. This is the first study to analyze RBCs membrane
charges during storage. In addition, we herein also measured the
elasticity of RBCs also collected in CPD-SAGM. In conclusion, the zeta
potential decreased 42% and cells were 134% less deformable at the end
of storage. The zeta potential from leukodepleted units had a similar
profile when compared to units stored without leukoreduction, indicating
that leukocyte lyses were not responsible for the zeta potential decay.
Flow cytometry measurements of reactive oxygen species suggested that
this decay is due to membrane oxidative damages. These results show that
measurements of zeta potentials provide new insights about RBCs storage
lesion for transfusion purposes.
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