%0 Journal Article %1 Wang2017Biosynthesis %A Wang, Meng %A Nie, Kaili %A Cao, Hao %A Xu, Haijun %A Fang, Yunming %A Tan, Tianwei %A Baeyens, Jan %A Liu, Luo %D 2017 %J Bioresource technology %K biofuels jatropha metabolic-engineering %P 542--545 %T Biosynthesis of medium chain length alkanes for bio-aviation fuel by metabolic engineered Escherichia coli. %U http://view.ncbi.nlm.nih.gov/pubmed/28550989 %V 239 %X The aim of this work was to study the synthesis of medium-chain length alkanes (MCLA), as bio-aviation product. To control the chain length of alkanes and increase the production of MCLA, Escherichia coli cells were engineered by incorporating (i) a chain length specific thioesterase from Umbellularia californica (UC), (ii) a plant origin acyl carrier protein (ACP) gene and (iii) the whole fatty acid synthesis system (FASs) from Jatropha curcas (JC). The genetic combination was designed to control the product spectrum towards optimum MCLA. Decanoic, lauric and myristic acid were produced at concentrations of 0.011, 0.093 and 1.657mg/g, respectively. The concentration of final products nonane, undecane and tridecane were 0.00062mg/g, 0.0052mg/g, and 0.249mg/g respectively. Thioesterase from UC controlled the fatty acid chain length in a range of 10-14 carbons and the ACP gene with whole FASs from JC significantly increased the production of MCLA. Copyright \copyright 2017 Elsevier Ltd. All rights reserved.

@article{Wang2017Biosynthesis, abstract = {The aim of this work was to study the synthesis of medium-chain length alkanes ({MCLA}), as bio-aviation product. To control the chain length of alkanes and increase the production of {MCLA}, Escherichia coli cells were engineered by incorporating (i) a chain length specific thioesterase from Umbellularia californica ({UC}), (ii) a plant origin acyl carrier protein ({ACP}) gene and (iii) the whole fatty acid synthesis system ({FASs}) from Jatropha curcas ({JC}). The genetic combination was designed to control the product spectrum towards optimum {MCLA}. Decanoic, lauric and myristic acid were produced at concentrations of 0.011, 0.093 and 1.657mg/g, respectively. The concentration of final products nonane, undecane and tridecane were 0.00062mg/g, 0.0052mg/g, and 0.249mg/g respectively. Thioesterase from {UC} controlled the fatty acid chain length in a range of 10-14 carbons and the {ACP} gene with whole {FASs} from {JC} significantly increased the production of {MCLA}. Copyright {\copyright} 2017 Elsevier Ltd. All rights reserved.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Wang, Meng and Nie, Kaili and Cao, Hao and Xu, Haijun and Fang, Yunming and Tan, Tianwei and Baeyens, Jan and Liu, Luo}, biburl = {https://www.bibsonomy.org/bibtex/2b1e56001cce6c85e6420d632d9dbf3b2/karthikraman}, citeulike-article-id = {14379766}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/28550989}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=28550989}, interhash = {b0b16f65aadfbeff48e88c41de452d00}, intrahash = {b1e56001cce6c85e6420d632d9dbf3b2}, issn = {1873-2976}, journal = {Bioresource technology}, keywords = {biofuels jatropha metabolic-engineering}, month = sep, pages = {542--545}, pmid = {28550989}, posted-at = {2017-06-20 16:42:13}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Biosynthesis of medium chain length alkanes for bio-aviation fuel by metabolic engineered Escherichia coli.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/28550989}, volume = 239, year = 2017 }