%0 Journal Article %1 citeulike:468437 %A Chen, J. %A Yan, F. %A Dai, Z. %A Ju, H. %C Key Laboratory of Analytical Chemistry for Life Science, Education Ministry of China, Department of Chemistry, Nanjing University, Nanjing 210093, PR China. %D 2005 %J Biosens Bioelectron %K hcg immunoassay homogeneous %N 2 %P 330--336 %R 10.1016/j.bios.2004.10.023 %T Reagentless amperometric immunosensor for human chorionic gonadotrophin based on direct electrochemistry of horseradish peroxidase. %U http://dx.doi.org/10.1016/j.bios.2004.10.023 %V 21 %X A novel amperometric immunosensor for determination of human serum chorionic gonadotrophin (HCG) was constructed by immobilization of HCG with titania sol-gel on a glassy carbon electrode and the direct electrochemistry of horseradish peroxidase (HRP) labeled to HCG antibody (HRP-anti-HCG). The morphologies of the HCG membrane were characterized to be chemically clean, porous and homogeneous. HRP-anti-HCG was functionally conjugated with the immobilized HCG after incubation in phosphate buffer (PBS) containing HRP-anti-HCG. A direct electron transfer of HRP with a rate constant of 1.35+/-0.40s(-1) was observed at the HRP-anti-HCG-HCG/titania sol-gel membrane modified electrode in 0.1M PBS pH 7.0. With a competitive mechanism the differential pulse voltammetric peak current of the immobilized HRP decreased linearly with an increasing HCG concentration from 2.5 to 12.5mIU/ml in the incubation solution. The HCG immunosensor showed a detection limit of 1.4mIU/ml, a good accuracy and acceptable precision and reproducibility with an intra-assay CV of 4.7% at 5.0mIU/ml and an inter-assay precision of 8.1% obtained at 10mIU/ml. The biosensor displayed a good stability in a storage period of 30 days.

@article{citeulike:468437, abstract = {A novel amperometric immunosensor for determination of human serum chorionic gonadotrophin (HCG) was constructed by immobilization of HCG with titania sol-gel on a glassy carbon electrode and the direct electrochemistry of horseradish peroxidase (HRP) labeled to HCG antibody (HRP-anti-HCG). The morphologies of the HCG membrane were characterized to be chemically clean, porous and homogeneous. HRP-anti-HCG was functionally conjugated with the immobilized HCG after incubation in phosphate buffer (PBS) containing HRP-anti-HCG. A direct electron transfer of HRP with a rate constant of 1.35+/-0.40s(-1) was observed at the HRP-anti-HCG-HCG/titania sol-gel membrane modified electrode in 0.1M PBS pH 7.0. With a competitive mechanism the differential pulse voltammetric peak current of the immobilized HRP decreased linearly with an increasing HCG concentration from 2.5 to 12.5mIU/ml in the incubation solution. The HCG immunosensor showed a detection limit of 1.4mIU/ml, a good accuracy and acceptable precision and reproducibility with an intra-assay CV of 4.7% at 5.0mIU/ml and an inter-assay precision of 8.1% obtained at 10mIU/ml. The biosensor displayed a good stability in a storage period of 30 days.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Key Laboratory of Analytical Chemistry for Life Science, Education Ministry of China, Department of Chemistry, Nanjing University, Nanjing 210093, PR China.}, author = {Chen, J. and Yan, F. and Dai, Z. and Ju, H.}, biburl = {https://www.bibsonomy.org/bibtex/25722b7b827132fd66ba37d5dd94cd197/biblio24}, citeulike-article-id = {468437}, doi = {10.1016/j.bios.2004.10.023}, interhash = {ba8f617455a62f81e65915b6b820159f}, intrahash = {5722b7b827132fd66ba37d5dd94cd197}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {hcg immunoassay homogeneous}, month = {August}, number = 2, pages = {330--336}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Reagentless amperometric immunosensor for human chorionic gonadotrophin based on direct electrochemistry of horseradish peroxidase.}, url = {http://dx.doi.org/10.1016/j.bios.2004.10.023}, volume = 21, year = 2005 }