%0 Journal Article %1 citeulike:7479718 %A Kim, Jeongyeon %A Song, Beomjong %A Hong, Ingie %A Kim, Jihye %A Lee, Junuk %A Park, Sungmo %A Eom, Jae Yong %A Lee, C. Justin %A Lee, Sukwon %A Choi, Sukwoo %D 2010 %J J. Neurosci. %K memory %N 28 %P 9631--9640 %R 10.1523/JNEUROSCI.0940-10.2010 %T Reactivation of Fear Memory Renders Consolidated Amygdala Synapses Labile %U http://dx.doi.org/10.1523/JNEUROSCI.0940-10.2010 %V 30 %X It is believed that memory reactivation transiently renders consolidated memory labile and that this labile or deconsolidated memory is reconsolidated in a protein synthesis-dependent manner. The synaptic correlate of memory deconsolidation upon reactivation, however, has not been fully characterized. Here, we show that 3,5-dihydroxyphenylglycine (DHPG), an agonist for group I metabotropic glutamate receptors (mGluRI), induces synaptic depotentiation only at thalamic input synapses onto the lateral amygdala (T-LA synapses) where synaptic potentiation is consolidated, but not at synapses where synaptic potentiation is not consolidated. Using this mGluRI-induced synaptic depotentiation (mGluRI-depotentiation) as a marker of consolidated synapses, we found that mGluRI-depotentiation correlated well with the state of memory deconsolidation and reconsolidation in a predictable manner. DHPG failed to induce mGluRI-depotentiation in slices prepared immediately after reactivation when the reactivated memory was deconsolidated. DHPG induced mGluRI-depotentiation 1 h after reactivation when the reactivated memory was reconsolidated, but it failed to do so when reconsolidation was blocked by a protein synthesis inhibitor. To test the memory-specificity of mGluRI-depotentiation, conditioned fear was acquired twice using two discriminative tones (2.8 and 20 kHz). Under this condition, mGluRI-depotentiation was fully impaired in slices prepared immediately after reactivation with both tones, whereas mGluRI-depotentiation was partially impaired immediately after reactivation with the 20 kHz tone. Consistently, microinjection of DHPG into the LA 1 h after reactivation reduced fear memory retention, whereas DHPG injection immediately after reactivation failed to do so. Our findings suggest that, upon memory reactivation, consolidated T-LA synapses enter a temporary labile state, displaying insensitivity to mGluRI-depotentiation. 10.1523/JNEUROSCI.0940-10.2010

@article{citeulike:7479718, abstract = {{It is believed that memory reactivation transiently renders consolidated memory labile and that this labile or deconsolidated memory is reconsolidated in a protein synthesis-dependent manner. The synaptic correlate of memory deconsolidation upon reactivation, however, has not been fully characterized. Here, we show that 3,5-dihydroxyphenylglycine (DHPG), an agonist for group I metabotropic glutamate receptors (mGluRI), induces synaptic depotentiation only at thalamic input synapses onto the lateral amygdala (T-LA synapses) where synaptic potentiation is consolidated, but not at synapses where synaptic potentiation is not consolidated. Using this mGluRI-induced synaptic depotentiation (mGluRI-depotentiation) as a marker of consolidated synapses, we found that mGluRI-depotentiation correlated well with the state of memory deconsolidation and reconsolidation in a predictable manner. DHPG failed to induce mGluRI-depotentiation in slices prepared immediately after reactivation when the reactivated memory was deconsolidated. DHPG induced mGluRI-depotentiation 1 h after reactivation when the reactivated memory was reconsolidated, but it failed to do so when reconsolidation was blocked by a protein synthesis inhibitor. To test the memory-specificity of mGluRI-depotentiation, conditioned fear was acquired twice using two discriminative tones (2.8 and 20 kHz). Under this condition, mGluRI-depotentiation was fully impaired in slices prepared immediately after reactivation with both tones, whereas mGluRI-depotentiation was partially impaired immediately after reactivation with the 20 kHz tone. Consistently, microinjection of DHPG into the LA 1 h after reactivation reduced fear memory retention, whereas DHPG injection immediately after reactivation failed to do so. Our findings suggest that, upon memory reactivation, consolidated T-LA synapses enter a temporary labile state, displaying insensitivity to mGluRI-depotentiation. 10.1523/JNEUROSCI.0940-10.2010}}, added-at = {2010-11-30T22:39:03.000+0100}, author = {Kim, Jeongyeon and Song, Beomjong and Hong, Ingie and Kim, Jihye and Lee, Junuk and Park, Sungmo and Eom, Jae Yong and Lee, C. Justin and Lee, Sukwon and Choi, Sukwoo}, biburl = {https://www.bibsonomy.org/bibtex/2a1096daf094cd4187efbfe2d6c128d13/smatthiesen}, citeulike-article-id = {7479718}, citeulike-linkout-0 = {http://dx.doi.org/10.1523/JNEUROSCI.0940-10.2010}, citeulike-linkout-1 = {http://www.jneurosci.org/cgi/content/abstract/30/28/9631}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/20631192}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=20631192}, day = 14, doi = {10.1523/JNEUROSCI.0940-10.2010}, interhash = {bcbb25d9dadf02ce0c279248345668c4}, intrahash = {a1096daf094cd4187efbfe2d6c128d13}, journal = {J. Neurosci.}, keywords = {memory}, month = {July}, number = 28, pages = {9631--9640}, posted-at = {2010-07-14 23:01:21}, priority = {2}, timestamp = {2010-11-30T22:39:12.000+0100}, title = {{Reactivation of Fear Memory Renders Consolidated Amygdala Synapses Labile}}, url = {http://dx.doi.org/10.1523/JNEUROSCI.0940-10.2010}, volume = 30, year = 2010 }