%0 Journal Article %1 Heidbreder2010 %A Heidbreder, Meike %A Endesfelder, Ulrike %A van de Linde, Sebastian %A Hennig, Simon %A Widera, Darius %A Kaltschmidt, Barbara %A Kaltschmidt, Christian %A Heilemann, Mike %C PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS %D 2010 %I ELSEVIER SCIENCE BV %J BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH %K linde mike %N 10 %P 1224-1229 %T Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots %U http://dx.doi.org/10.1016/j.bbamcr.2010.06.004 %V 1803 %X We introduce semiconductor quantum dot-based fluorescence imaging with similar to 2-fold increased optical resolution in three dimensions as a method that allows both studying cellular structures and spatial organization of biomolecules in membranes and subcellular organelles. Target biomolecules are labelled with quantum dots via immunocytochemistry. The resolution enhancement is achieved by three-photon absorption of quantum dots and subsequent fluorescence emission from a higher-order excitonic state. Different from conventional multiphoton microscopy, this approach can be realized on any confocal microscope without the need for pulsed excitation light. We demonstrate quantum dot triexciton imaging (QDTI) of the microtubule network of U373 cells, 3D imaging of TNF receptor 2 on the plasma membrane of HeLa cells, and multicolor 3D imaging of mitochondrial cytochrome c oxidase and actin in COS-7 cells. (C) 2010 Elsevier B.V. All rights reserved.

@article{Heidbreder2010, abstract = {We introduce semiconductor quantum dot-based fluorescence imaging with similar to 2-fold increased optical resolution in three dimensions as a method that allows both studying cellular structures and spatial organization of biomolecules in membranes and subcellular organelles. Target biomolecules are labelled with quantum dots via immunocytochemistry. The resolution enhancement is achieved by three-photon absorption of quantum dots and subsequent fluorescence emission from a higher-order excitonic state. Different from conventional multiphoton microscopy, this approach can be realized on any confocal microscope without the need for pulsed excitation light. We demonstrate quantum dot triexciton imaging (QDTI) of the microtubule network of U373 cells, 3D imaging of TNF receptor 2 on the plasma membrane of HeLa cells, and multicolor 3D imaging of mitochondrial cytochrome c oxidase and actin in COS-7 cells. (C) 2010 Elsevier B.V. All rights reserved.}, added-at = {2011-03-04T10:38:08.000+0100}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, affiliation = {Heilemann, M (Reprint Author), Univ Bielefeld, Dept Phys, Univ Str 25, D-33615 Bielefeld, Germany. {[}Heidbreder, Meike; Endesfelder, Ulrike; van de Linde, Sebastian; Hennig, Simon; Heilemann, Mike] Univ Bielefeld, Dept Phys, D-33615 Bielefeld, Germany. {[}Kaltschmidt, Barbara] Univ Bielefeld, Dept Biol, D-33615 Bielefeld, Germany.}, author = {Heidbreder, Meike and Endesfelder, Ulrike and van de Linde, Sebastian and Hennig, Simon and Widera, Darius and Kaltschmidt, Barbara and Kaltschmidt, Christian and Heilemann, Mike}, author-email = {heileman@physik.uni-bielefeld.de}, biburl = {https://www.bibsonomy.org/bibtex/2f3ffb455d483301435ab8d550ba3e7af/reichert}, doc-delivery-number = {651IA}, funding-acknowledgement = {Systems Biology Initiative (FORSYS) of the German Ministry of Research and Education (BMBF) {[}0315262]}, funding-text = {This work was supported by the Systems Biology Initiative (FORSYS) of the German Ministry of Research and Education (BMBF, grant 0315262).}, groups = {public}, interhash = {e82514cb9eef3ceacbcd950fa24e3bcd}, intrahash = {f3ffb455d483301435ab8d550ba3e7af}, issn = {0167-4889}, journal = {BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH}, journal-iso = {Biochim. Biophys. Acta-Mol. Cell Res.}, keywords = {linde mike}, language = {English}, month = OCT, number = 10, number-of-cited-references = {24}, pages = {1224-1229}, publisher = {ELSEVIER SCIENCE BV}, subject-category = {Biochemistry \& Molecular Biology; Cell Biology}, times-cited = {0}, timestamp = {2011-06-29T14:29:37.000+0200}, title = {Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots}, type = {Article}, unique-id = {ISI:000281920200011}, url = {http://dx.doi.org/10.1016/j.bbamcr.2010.06.004}, username = {reichert}, volume = 1803, year = 2010 }