%0 Journal Article %1 citeulike:558743 %A Fruchart, J. C. %A Desreumaux, C. %A Dewailly, P. %A Sezille, G. %A Jaillard, J. %A Carlier, Y. %A Bout, D. %A Capron, A. %D 1978 %J Clin Chem %K competition immunoassay elisa apob %N 3 %P 455--459 %T Enzyme immunoassay for human apolipoprotein B, the major protein moiety in low-density- and very-low-density lipoproteins. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=204430 %V 24 %X We used enzyme immunoassay to measure apolipoprotein B concentration in human plasma. Pure lipoprotein B was isolated from serum samples of fasting normolipidemic subjects by sequential preparative ultracentrifugation and coated to a polystyrene tube surface by adsorption. Human serum samples and rabbit antiserum to human apolipoprotein B were incubated with the solid-phase lipoprotein B. Soluble antigen competed with solid-phase antigen for binding to antibodies. After washing, peroxidase-labeled sheep antibodies against rabbit immunoglobulins were added, and after further washing the bound label was assayed. This provided a direct measurement of the soluble antigen. The best technical conditions for the assay were determined. The minimum detectable concentration was 1 microgram per assay. The enzyme immunoassay yielded values that compare favorably with those obtained by radial immunodiffusion (r = 0.84) and by rocket immunoelectrophoresis (r = 0.80). The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, ane non-use of radioisotopes.

@article{citeulike:558743, abstract = {We used enzyme immunoassay to measure apolipoprotein B concentration in human plasma. Pure lipoprotein B was isolated from serum samples of fasting normolipidemic subjects by sequential preparative ultracentrifugation and coated to a polystyrene tube surface by adsorption. Human serum samples and rabbit antiserum to human apolipoprotein B were incubated with the solid-phase lipoprotein B. Soluble antigen competed with solid-phase antigen for binding to antibodies. After washing, peroxidase-labeled sheep antibodies against rabbit immunoglobulins were added, and after further washing the bound label was assayed. This provided a direct measurement of the soluble antigen. The best technical conditions for the assay were determined. The minimum detectable concentration was 1 microgram per assay. The enzyme immunoassay yielded values that compare favorably with those obtained by radial immunodiffusion (r = 0.84) and by rocket immunoelectrophoresis (r = 0.80). The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, ane non-use of radioisotopes.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Fruchart, J. C. and Desreumaux, C. and Dewailly, P. and Sezille, G. and Jaillard, J. and Carlier, Y. and Bout, D. and Capron, A.}, biburl = {https://www.bibsonomy.org/bibtex/28ae681e108fad050944c1b7b76dc1260/biblio24}, citeulike-article-id = {558743}, interhash = {ecdac005612f9cc1d6ba084746a4ad70}, intrahash = {8ae681e108fad050944c1b7b76dc1260}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {competition immunoassay elisa apob}, month = {March}, number = 3, pages = {455--459}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Enzyme immunoassay for human apolipoprotein B, the major protein moiety in low-density- and very-low-density lipoproteins.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=204430}, volume = 24, year = 1978 }