%0 Journal Article %1 Nomura.1995 %A Nomura, M. %A Yamagishi, S. %A Harada, S. %A Hayashi, Y. %A Yamashima, T. %A Yamashita, J. %A Yamamoto, H. %D 1995 %J J.Biol.Chem. %K & A Animals Antisense Base Cattle Cell Cells Chain Cultured DNA Data Division Dna Endothelial Endothelium Factor Factors Growth Human Humans Hypoxia Japan Kinase Kinases Kinetics Lymphokines Messenger Microcirculation Mitogen Molecular Muscle Oligonucleotide Oligonucleotides Oxygen Polymerase Primers Probes Protein-Tyrosine Proteins Proto-Oncogene RNA Reaction Receptor Receptor-1 Receptors Replication Research Retina Retinal Sequence Smooth Tyrosine Umbilical Vascular Veins Vessels analysis antagonists biosynthesis cells cytology drug effects inhibitors pharmacology physiology protein %N 47 %P 28316-28324 %T Possible participation of autocrine and paracrine vascular endothelial growth factors in hypoxia-induced proliferation of endothelial cells and pericytes %U PM:7499331 %V 270 %X Hypoxia is the principal factor that causes angiogenesis. These experiments were conducted to explore how it induces the proliferation of vascular cells, a key step in angiogenesis. Human umbilical vein endothelial cells and bovine retinal pericytes were grown in controlled atmosphere culture chambers containing various concentrations of oxygen. The numbers of both endothelial cells and pericytes increased significantly under hypoxic conditions; the O2 concentrations that achieved maximal growth promotion were 10% for endothelial cells and 2.5% for pericytes. Quantitative reverse transcription-polymerase chain reaction analysis revealed that mRNAs coding for the secretory forms of vascular endothelial growth factor (VEGF), a mitogen specific to endothelial cells, were present in both endothelial cells and pericytes and that their levels increased significantly in the two cell types as the atmospheric O2 concentration decreased. The two genes for VEGF receptors, kinase insert domain-con

@article{Nomura.1995, abstract = {Hypoxia is the principal factor that causes angiogenesis. These experiments were conducted to explore how it induces the proliferation of vascular cells, a key step in angiogenesis. Human umbilical vein endothelial cells and bovine retinal pericytes were grown in controlled atmosphere culture chambers containing various concentrations of oxygen. The numbers of both endothelial cells and pericytes increased significantly under hypoxic conditions; the O2 concentrations that achieved maximal growth promotion were 10% for endothelial cells and 2.5% for pericytes. Quantitative reverse transcription-polymerase chain reaction analysis revealed that mRNAs coding for the secretory forms of vascular endothelial growth factor (VEGF), a mitogen specific to endothelial cells, were present in both endothelial cells and pericytes and that their levels increased significantly in the two cell types as the atmospheric O2 concentration decreased. The two genes for VEGF receptors, kinase insert domain-con}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Nomura, M. and Yamagishi, S. and Harada, S. and Hayashi, Y. and Yamashima, T. and Yamashita, J. and Yamamoto, H.}, biburl = {https://www.bibsonomy.org/bibtex/27264a9daea670265d8348e986eeb2b5f/kanefendt}, interhash = {1f420deb9e4edfb2a8dab2705d86eb04}, intrahash = {7264a9daea670265d8348e986eeb2b5f}, journal = {J.Biol.Chem.}, keywords = {& A Animals Antisense Base Cattle Cell Cells Chain Cultured DNA Data Division Dna Endothelial Endothelium Factor Factors Growth Human Humans Hypoxia Japan Kinase Kinases Kinetics Lymphokines Messenger Microcirculation Mitogen Molecular Muscle Oligonucleotide Oligonucleotides Oxygen Polymerase Primers Probes Protein-Tyrosine Proteins Proto-Oncogene RNA Reaction Receptor Receptor-1 Receptors Replication Research Retina Retinal Sequence Smooth Tyrosine Umbilical Vascular Veins Vessels analysis antagonists biosynthesis cells cytology drug effects inhibitors pharmacology physiology protein}, number = 47, pages = {28316-28324}, timestamp = {2010-02-05T11:28:44.000+0100}, title = {Possible participation of autocrine and paracrine vascular endothelial growth factors in hypoxia-induced proliferation of endothelial cells and pericytes}, url = {PM:7499331}, volume = 270, year = 1995 }