%0 Journal Article %1 Chen2015Emergent %A Chen, Ye %A Kim, Jae K. %A Hirning, Andrew J. %A Josić, Kresimir %A Bennett, Matthew R. %D 2015 %I American Association for the Advancement of Science %J Science %K consortia oscillators synthetic-biology %N 6251 %P 986--989 %R 10.1126/science.aaa3794 %T Emergent genetic oscillations in a synthetic microbial consortium %U http://dx.doi.org/10.1126/science.aaa3794 %V 349 %X A challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types—an äctivator" strain and a "repressor" strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types. Copyright \copyright 2015, American Association for the Advancement of Science.

@article{Chen2015Emergent, abstract = {A challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types—an "activator" strain and a "repressor" strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types. Copyright {\copyright} 2015, American Association for the Advancement of Science.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Chen, Ye and Kim, Jae K. and Hirning, Andrew J. and Josi\'{c}, Kre\v{s}imir and Bennett, Matthew R.}, biburl = {https://www.bibsonomy.org/bibtex/2a36f1626a8b97839e191f503d3823793/karthikraman}, citeulike-article-id = {13714226}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.aaa3794}, citeulike-linkout-1 = {http://www.sciencemag.org/content/349/6251/986.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/349/6251/986.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/26315440}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=26315440}, day = 28, doi = {10.1126/science.aaa3794}, interhash = {f45ee4a58b88c52d8aaf061196058675}, intrahash = {a36f1626a8b97839e191f503d3823793}, issn = {1095-9203}, journal = {Science}, keywords = {consortia oscillators synthetic-biology}, month = aug, number = 6251, pages = {986--989}, pmid = {26315440}, posted-at = {2015-11-03 06:16:41}, priority = {2}, publisher = {American Association for the Advancement of Science}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Emergent genetic oscillations in a synthetic microbial consortium}, url = {http://dx.doi.org/10.1126/science.aaa3794}, volume = 349, year = 2015 }