%0 Journal Article %1 herbell2018interconnection %A Herbell, S. %A Gutermuth, T. %A Konrad, K. R. %D 2018 %J Plant Signal Behav %K Anions/*metabolism myOwn %N 11 %P e1529521 %R 10.1080/15592324.2018.1529521 %T An interconnection between tip-focused Ca(2+) and anion homeostasis controls pollen tube growth %U https://www.ncbi.nlm.nih.gov/pubmed/30307369 %V 13 %X Plant reproduction is the basis for economically relevant food production. It relies on pollen tube (PTs) growth into the female flower organs for successful fertilization. The high cytosolic Ca(2+) concentration (Ca(2+)(cyt)) at the PT tip is sensed by Ca(2+)-dependent protein kinases (CPKs) that in turn activate R- and S-type anion channels to control polar growth. Lanthanum, a blocker for plant Ca(2+)-permeable channels was used here to demonstrate a strict dependency for anion channel activation through high PT tip Ca(2+)(cyt). We visualized this relationship by live-cell anion imaging and concurrent triggering of Ca(2+)-elevations with the two-electrode voltage-clamp (TEVC) technique. The anion efflux provoked by a TEVC-triggered Ca(2+)(cyt) increase was abolished by Lanthanum and was followed by an overall rise in the cytosolic anion concentration. An interrelation between Ca(2+) and anion homeostasis occurred also on the transcript level of CPKs and anion channels. qRT-PCR analysis demonstrated a co-regulation of anion channels and CPKs in media with different Cl(-) and NO(3)(-) compositions. Our data provides strong evidence for the importance of a Ca(2+)-dependent anion channel regulation and point to a synchronized adjustment of CPK and anion channel transcript levels to fine-tune anion efflux at the PT tip.

@article{herbell2018interconnection, abstract = {Plant reproduction is the basis for economically relevant food production. It relies on pollen tube (PTs) growth into the female flower organs for successful fertilization. The high cytosolic Ca(2+) concentration ([Ca(2+)](cyt)) at the PT tip is sensed by Ca(2+)-dependent protein kinases (CPKs) that in turn activate R- and S-type anion channels to control polar growth. Lanthanum, a blocker for plant Ca(2+)-permeable channels was used here to demonstrate a strict dependency for anion channel activation through high PT tip [Ca(2+)](cyt). We visualized this relationship by live-cell anion imaging and concurrent triggering of Ca(2+)-elevations with the two-electrode voltage-clamp (TEVC) technique. The anion efflux provoked by a TEVC-triggered [Ca(2+)](cyt) increase was abolished by Lanthanum and was followed by an overall rise in the cytosolic anion concentration. An interrelation between Ca(2+) and anion homeostasis occurred also on the transcript level of CPKs and anion channels. qRT-PCR analysis demonstrated a co-regulation of anion channels and CPKs in media with different Cl(-) and NO(3)(-) compositions. Our data provides strong evidence for the importance of a Ca(2+)-dependent anion channel regulation and point to a synchronized adjustment of CPK and anion channel transcript levels to fine-tune anion efflux at the PT tip.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Herbell, S. and Gutermuth, T. and Konrad, K. R.}, biburl = {https://www.bibsonomy.org/bibtex/2458b5f8ce9232de8813d2e810610a9ee/jvsi_all}, doi = {10.1080/15592324.2018.1529521}, interhash = {fe7e8fc5c308d4db7365caa3accad82a}, intrahash = {458b5f8ce9232de8813d2e810610a9ee}, issn = {1559-2324 (Electronic) 1559-2316 (Print) 1559-2316 (Linking)}, journal = {Plant Signal Behav}, keywords = {Anions/*metabolism myOwn}, note = {Herbell, Sarah Gutermuth, Timo Konrad, Kai Robert eng Research Support, Non-U.S. Gov't 2018/10/12 Plant Signal Behav. 2018;13(11):e1529521. doi: 10.1080/15592324.2018.1529521. Epub 2018 Oct 11.}, number = 11, pages = {e1529521}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {An interconnection between tip-focused Ca(2+) and anion homeostasis controls pollen tube growth}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/30307369}, volume = 13, year = 2018 }