%0 Journal Article %1 citeulike:470433 %A Haga, Makoto %A Itagaki, Hiroshi %A Sugawara, Shinya %A Okano, Teisuke %D 1980 %J Biochemical and Biophysical Research Communications %K l-i-l-a competition complement immunoassay homogeneous ag_detection liposome %N 1 %P 187--192 %R 10.1016/0006-291X(80)90722-6 %T Liposome immunosensor for theophylline %U http://www.sciencedirect.com/science/article/B6WBK-4DN95M1-YT/2/33237bbe4fae6c1970b4df80bd1687c1 %V 95 %X A new simple, sensitive liposome immunosensor (LIS) has been developed by combining the advantages of spin membrane immunoassay (SMIA) and enzyme immunosensor (EIS). The LIS system is composed of an oxygen electrode and sensitized liposomes. It records liposome lysis induced by specific anti-theophylline antibodies and complement which is monitored by the release of entrapped enzymes instead of spin labeles. A sensitive detection was performed because of the amplification of antigen-antibody reaction by liposome lysis and enzymatic reaction. The method offers a simple and sensitive quantitative detection of theophylline down to 4 x 10-9 M (0.7 ng/ml).

@article{citeulike:470433, abstract = {A new simple, sensitive liposome immunosensor (LIS) has been developed by combining the advantages of spin membrane immunoassay (SMIA) and enzyme immunosensor (EIS). The LIS system is composed of an oxygen electrode and sensitized liposomes. It records liposome lysis induced by specific anti-theophylline antibodies and complement which is monitored by the release of entrapped enzymes instead of spin labeles. A sensitive detection was performed because of the amplification of antigen-antibody reaction by liposome lysis and enzymatic reaction. The method offers a simple and sensitive quantitative detection of theophylline down to 4 x 10-9 M (0.7 ng/ml).}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Haga, Makoto and Itagaki, Hiroshi and Sugawara, Shinya and Okano, Teisuke}, biburl = {https://www.bibsonomy.org/bibtex/20d6507754eff710179f6547d81beb8f5/biblio24}, citeulike-article-id = {470433}, doi = {10.1016/0006-291X(80)90722-6}, interhash = {2074e5018e25c49043e0caa8069870b8}, intrahash = {0d6507754eff710179f6547d81beb8f5}, journal = {Biochemical and Biophysical Research Communications}, keywords = {l-i-l-a competition complement immunoassay homogeneous ag_detection liposome}, month = {July}, number = 1, pages = {187--192}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Liposome immunosensor for theophylline}, url = {http://www.sciencedirect.com/science/article/B6WBK-4DN95M1-YT/2/33237bbe4fae6c1970b4df80bd1687c1}, volume = 95, year = 1980 }