An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).
%0 Journal Article
%1 citeulike:470384
%A López, M. A.
%A Ortega, F.
%A Domínguez, E.
%A Katakis, I.
%C Departamento de Química Analítica, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
%D 1998
%J J Mol Recognit
%K amperometry redox_polymers immunoassay homogeneous ag_detection electrochemistry immunoelectrode
%N 1-6
%P 178--181
%T Electrochemical immunosensor for the detection of atrazine.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10076834
%V 11
%X An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).
@article{citeulike:470384,
abstract = {An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).},
added-at = {2006-07-07T01:10:50.000+0200},
address = {Departamento de Química Analítica, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.},
author = {López, M. A. and Ortega, F. and Domínguez, E. and Katakis, I.},
biburl = {https://www.bibsonomy.org/bibtex/20fd6c1fa198c2ca77bf510de53fa1228/biblio24},
citeulike-article-id = {470384},
comment = {qadominguez@inqui.alcala.es},
interhash = {25741c8b06dddb9ad01c800c5bbfd38d},
intrahash = {0fd6c1fa198c2ca77bf510de53fa1228},
issn = {0952-3499},
journal = {J Mol Recognit},
keywords = {amperometry redox_polymers immunoassay homogeneous ag_detection electrochemistry immunoelectrode},
number = {1-6},
pages = {178--181},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Electrochemical immunosensor for the detection of atrazine.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=10076834},
volume = 11,
year = 1998
}