%0 Journal Article %1 davies1993exopolysaccharide %A Davies, D G %A Chakrabarty, A M %A Geesey, G G %D 1993 %J Applied and Environmental Microbiology %K 92c70-microbiology biofilm %N 4 %P 1181-1186 %R 10.1128/aem.59.4.1181-1186.1993 %T Exopolysaccharide production in biofilms: substratum activation of alginate gene expression by Pseudomonas aeruginosa %U https://journals.asm.org/doi/10.1128/aem.59.4.1181-1186.1993 %V 59 %X Reporter gene technology was employed to detect the activity of an alginate promoter of Pseudomonas aeruginosa when the organism was grown as a biofilm on a Teflon mesh substratum and as planktonic cells in liquid medium. Alginate biosynthetic activity was determined with a mucoid cell line derived from a cystic fibrosis isolate and containing an alginate algC promoter fused to a lacZ reporter gene. Reporter activity was demonstrated with chromogenic and fluorogenic substrates for beta-galactosidase. Expression of algC was shown to be upregulated in biofilm cells compared with planktonic cells in liquid medium. Gene up-expression correlated with alginate biosynthesis as measured by Fourier transform infrared spectroscopy, uronic acid accumulation, and alginate-specific enzyme-linked immunosorbent assay. The algC promoter was shown to have maximum activity in planktonic cultures during the late lag and early log phases of the cell growth cycle. During a time course experiment, biofilm algC activity exceeded planktonic activity except during the period immediately following inoculation into fresh medium. In continuous-culture experiments, conversion of lacZ substrate was demonstrated microscopically in individual cells by epifluorescence microscopy.

@article{davies1993exopolysaccharide, abstract = {Reporter gene technology was employed to detect the activity of an alginate promoter of Pseudomonas aeruginosa when the organism was grown as a biofilm on a Teflon mesh substratum and as planktonic cells in liquid medium. Alginate biosynthetic activity was determined with a mucoid cell line derived from a cystic fibrosis isolate and containing an alginate algC promoter fused to a lacZ reporter gene. Reporter activity was demonstrated with chromogenic and fluorogenic substrates for beta-galactosidase. Expression of algC was shown to be upregulated in biofilm cells compared with planktonic cells in liquid medium. Gene up-expression correlated with alginate biosynthesis as measured by Fourier transform infrared spectroscopy, uronic acid accumulation, and alginate-specific enzyme-linked immunosorbent assay. The algC promoter was shown to have maximum activity in planktonic cultures during the late lag and early log phases of the cell growth cycle. During a time course experiment, biofilm algC activity exceeded planktonic activity except during the period immediately following inoculation into fresh medium. In continuous-culture experiments, conversion of lacZ substrate was demonstrated microscopically in individual cells by epifluorescence microscopy.}, added-at = {2023-09-27T04:37:38.000+0200}, author = {Davies, D G and Chakrabarty, A M and Geesey, G G}, biburl = {https://www.bibsonomy.org/bibtex/2147fac5067847203ddec89cef7c3317f/gdmcbain}, doi = {10.1128/aem.59.4.1181-1186.1993}, interhash = {80946009163c1ba54320e29503f7d36b}, intrahash = {147fac5067847203ddec89cef7c3317f}, journal = {Applied and Environmental Microbiology}, keywords = {92c70-microbiology biofilm}, number = 4, pages = {1181-1186}, timestamp = {2023-09-27T04:39:56.000+0200}, title = {Exopolysaccharide production in biofilms: substratum activation of alginate gene expression by Pseudomonas aeruginosa}, url = {https://journals.asm.org/doi/10.1128/aem.59.4.1181-1186.1993}, volume = 59, year = 1993 }