Alkaloid Concentrations of Lolium perenne Infected with Epichloe festucae var. lolii with Different Detection Methods-A Re-Evaluation of Intoxication Risk in Germany?
V. Vikuk, B. Fuchs, M. Krischke, M. Mueller, S. Rueb, and J. Krauss. J Fungi (Basel), (2020)Vikuk, Veronika
Fuchs, Benjamin
Krischke, Markus
Mueller, Martin J
Rueb, Selina
Krauss, Jochen
eng
KR 3559/3-2/Deutsche Forschungsgemeinschaft/
Switzerland
2020/09/24
J Fungi (Basel). 2020 Sep 18;6(3):177. doi: 10.3390/jof6030177..
DOI: 10.3390/jof6030177
Abstract
Mycotoxins in agriculturally used plants can cause intoxication in animals and can lead to severe financial losses for farmers. The endophytic fungus Epichloe festucae var. lolii living symbiotically within the cool season grass species Lolium perenne can produce vertebrate and invertebrate toxic alkaloids. Hence, an exact quantitation of alkaloid concentrations is essential to determine intoxication risk for animals. Many studies use different methods to detect alkaloid concentrations, which complicates the comparability. In this study, we showed that alkaloid concentrations of individual plants exceeded toxicity thresholds on real world grasslands in Germany, but not on the population level. Alkaloid concentrations on five German grasslands with high alkaloid levels peaked in summer but were also below toxicity thresholds on population level. Furthermore, we showed that alkaloid concentrations follow the same seasonal trend, regardless of whether plant fresh or dry weight was used, in the field and in a common garden study. However, alkaloid concentrations were around three times higher when detected with dry weight. Finally, we showed that alkaloid concentrations can additionally be biased to different alkaloid detection methods. We highlight that toxicity risks should be analyzed using plant dry weight, but concentration trends of fresh weight are reliable.
%0 Journal Article
%1 vikuk2020alkaloid
%A Vikuk, V.
%A Fuchs, B.
%A Krischke, M.
%A Mueller, M. J.
%A Rueb, S.
%A Krauss, J.
%D 2020
%J J Fungi (Basel)
%K Epichloe myOwn uni_network
%N 3
%R 10.3390/jof6030177
%T Alkaloid Concentrations of Lolium perenne Infected with Epichloe festucae var. lolii with Different Detection Methods-A Re-Evaluation of Intoxication Risk in Germany?
%U https://www.ncbi.nlm.nih.gov/pubmed/32961967https://mdpi-res.com/d_attachment/jof/jof-06-00177/article_deploy/jof-06-00177-v2.pdf?version=1600695814
%V 6
%X Mycotoxins in agriculturally used plants can cause intoxication in animals and can lead to severe financial losses for farmers. The endophytic fungus Epichloe festucae var. lolii living symbiotically within the cool season grass species Lolium perenne can produce vertebrate and invertebrate toxic alkaloids. Hence, an exact quantitation of alkaloid concentrations is essential to determine intoxication risk for animals. Many studies use different methods to detect alkaloid concentrations, which complicates the comparability. In this study, we showed that alkaloid concentrations of individual plants exceeded toxicity thresholds on real world grasslands in Germany, but not on the population level. Alkaloid concentrations on five German grasslands with high alkaloid levels peaked in summer but were also below toxicity thresholds on population level. Furthermore, we showed that alkaloid concentrations follow the same seasonal trend, regardless of whether plant fresh or dry weight was used, in the field and in a common garden study. However, alkaloid concentrations were around three times higher when detected with dry weight. Finally, we showed that alkaloid concentrations can additionally be biased to different alkaloid detection methods. We highlight that toxicity risks should be analyzed using plant dry weight, but concentration trends of fresh weight are reliable.
@article{vikuk2020alkaloid,
abstract = {Mycotoxins in agriculturally used plants can cause intoxication in animals and can lead to severe financial losses for farmers. The endophytic fungus Epichloe festucae var. lolii living symbiotically within the cool season grass species Lolium perenne can produce vertebrate and invertebrate toxic alkaloids. Hence, an exact quantitation of alkaloid concentrations is essential to determine intoxication risk for animals. Many studies use different methods to detect alkaloid concentrations, which complicates the comparability. In this study, we showed that alkaloid concentrations of individual plants exceeded toxicity thresholds on real world grasslands in Germany, but not on the population level. Alkaloid concentrations on five German grasslands with high alkaloid levels peaked in summer but were also below toxicity thresholds on population level. Furthermore, we showed that alkaloid concentrations follow the same seasonal trend, regardless of whether plant fresh or dry weight was used, in the field and in a common garden study. However, alkaloid concentrations were around three times higher when detected with dry weight. Finally, we showed that alkaloid concentrations can additionally be biased to different alkaloid detection methods. We highlight that toxicity risks should be analyzed using plant dry weight, but concentration trends of fresh weight are reliable.},
added-at = {2024-02-15T15:11:54.000+0100},
author = {Vikuk, V. and Fuchs, B. and Krischke, M. and Mueller, M. J. and Rueb, S. and Krauss, J.},
biburl = {https://www.bibsonomy.org/bibtex/215d60659c0e74bbea6330f30b31b2a53/jvsi_all},
doi = {10.3390/jof6030177},
interhash = {4c6c5bf69d3023ce51e7a35a3addefbd},
intrahash = {15d60659c0e74bbea6330f30b31b2a53},
issn = {2309-608X (Electronic)
2309-608X (Linking)},
journal = {J Fungi (Basel)},
keywords = {Epichloe myOwn uni_network},
note = {Vikuk, Veronika
Fuchs, Benjamin
Krischke, Markus
Mueller, Martin J
Rueb, Selina
Krauss, Jochen
eng
KR 3559/3-2/Deutsche Forschungsgemeinschaft/
Switzerland
2020/09/24
J Fungi (Basel). 2020 Sep 18;6(3):177. doi: 10.3390/jof6030177.},
number = 3,
timestamp = {2024-02-15T15:11:54.000+0100},
title = {Alkaloid Concentrations of Lolium perenne Infected with Epichloe festucae var. lolii with Different Detection Methods-A Re-Evaluation of Intoxication Risk in Germany?},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/32961967https://mdpi-res.com/d_attachment/jof/jof-06-00177/article_deploy/jof-06-00177-v2.pdf?version=1600695814},
volume = 6,
year = 2020
}