%0 Journal Article %1 RajashekarBurke_96 %A Rajashekar, C. %A Burke, M. J. %D 1996 %J Plant Physiology %K bibtex-import, cellular, citeulikeExport cryo, freezing, ice, intracellular, physics, potential, snowgumpapermaybe, spread, tolerance, unentered, water %P 597--603 %T Freezing Characteristics of Rigid Plant Tissues %V 111 %X The results suggest that resistance to cell deformation during extracellular freezing due to cell-wall rigidity can lead to reduced cell dehydration and increased cell tension.

@article{RajashekarBurke_96, abstract = {{The results suggest that resistance to cell deformation during extracellular freezing due to cell-wall rigidity can lead to reduced cell dehydration and increased cell tension.}}, added-at = {2019-03-31T01:14:40.000+0100}, author = {Rajashekar, C. and Burke, M. J.}, biburl = {https://www.bibsonomy.org/bibtex/223dc19f09b80067fc3b4c93fce2fdfbd/dianella}, citeulike-article-id = {1523982}, comment = {(private-note)* 1.16MPa rule is from hereabouts Cells can be partially frozen and under tension The results suggest that resistance to cell deformation during extracellular freezing due to cell-wall rigidity can lead to reduced cell dehydration and increased cell tension. Highly theoretical NMR used to estimate freezing points, unfrozen water, Plots with temperature for unfreezable water Osmolality from melting point depression of samples and from expressed leaf sap. (Sounds like a dodgey method for getting sap) Frozen to LN2 temperature and then thawed!!! Extrapolated tension effects on nuclation temperature (more tension, homogeneous nucleation at higher temperatures) I don't understand the reasoning behind this: If cells are rigid enough, supercooling occurs If cells are less rigid, they'll cavitate (???) Check out Malone and Ashworth 1991 "Similarly, leaf cell tension reached a maximum of 8.3 MPa at approximately -25"C, followed by a sharp decrease with further cooling in leaves of mountain cranberry. Decline or loss of tension is generally attributed to cell-water cavitation (Henderson and Speedy, 1987), which is likely to cause cell injury or death. Henderson and Speedy (1987) also noted that cavitation of water at low temperatures was often associated with spontaneous ice formation, which may result in lethal injury to cells."}, interhash = {672f1d15d5e710d60be053a66f3294c6}, intrahash = {23dc19f09b80067fc3b4c93fce2fdfbd}, journal = {Plant Physiology}, keywords = {bibtex-import, cellular, citeulikeExport cryo, freezing, ice, intracellular, physics, potential, snowgumpapermaybe, spread, tolerance, unentered, water}, pages = {597--603}, posted-at = {2007-07-31 07:01:01}, priority = {2}, timestamp = {2019-03-31T01:16:26.000+0100}, title = {{Freezing Characteristics of Rigid Plant Tissues}}, volume = 111, year = 1996 }