%0 Journal Article %1 Mich_2005_2234 %A Michailova, Anushka %A Saucerman, Jeffrey %A Belik, Mary Ellen %A McCulloch, Andrew D %D 2005 %J Biophys J %K Adenosine Calcium Cardiac, Cardiovascular; Cells, Channel Channels, Computer Contraction, Cultured; Diphosphate, Gating, Homeostasis, Ion L-Type, Magnesium, Models, Myocardial Myocytes, Potassium Signal Signaling, Simulation; Transduction, Triphosphate, metabolism; physiology physiology; %N 3 %P 2234--2249 %R 10.1529/biophysj.104.046284 %T Modeling regulation of cardiac KATP and L-type Ca2+ currents by ATP, ADP, and Mg2+. %U http://dx.doi.org/10.1529/biophysj.104.046284 %V 88 %X Changes in cytosolic free Mg(2+) and adenosine nucleotide phosphates affect cardiac excitability and contractility. To investigate how modulation by Mg(2+), ATP, and ADP of K(ATP) and L-type Ca(2+) channels influences excitation-contraction coupling, we incorporated equations for intracellular ATP and MgADP regulation of the K(ATP) current and MgATP regulation of the L-type Ca(2+) current in an ionic-metabolic model of the canine ventricular myocyte. The new model: 1), quantitatively reproduces a dose-response relationship for the effects of changes in ATP on K(ATP) current, 2), simulates effects of ADP in modulating ATP sensitivity of K(ATP) channel, 3), predicts activation of Ca(2+) current during rapid increase in MgATP, and 4), demonstrates that decreased ATP/ADP ratio with normal total Mg(2+) or increased free Mg(2+) with normal ATP and ADP activate K(ATP) current, shorten action potential, and alter ionic currents and intracellular Ca(2+) signals. The model predictions are in agreement with experimental data measured under normal and a variety of pathological conditions.

@article{Mich_2005_2234, abstract = {Changes in cytosolic free Mg(2+) and adenosine nucleotide phosphates affect cardiac excitability and contractility. To investigate how modulation by Mg(2+), ATP, and ADP of K(ATP) and L-type Ca(2+) channels influences excitation-contraction coupling, we incorporated equations for intracellular ATP and MgADP regulation of the K(ATP) current and MgATP regulation of the L-type Ca(2+) current in an ionic-metabolic model of the canine ventricular myocyte. The new model: 1), quantitatively reproduces a dose-response relationship for the effects of changes in ATP on K(ATP) current, 2), simulates effects of ADP in modulating ATP sensitivity of K(ATP) channel, 3), predicts activation of Ca(2+) current during rapid increase in MgATP, and 4), demonstrates that decreased ATP/ADP ratio with normal total Mg(2+) or increased free Mg(2+) with normal ATP and ADP activate K(ATP) current, shorten action potential, and alter ionic currents and intracellular Ca(2+) signals. The model predictions are in agreement with experimental data measured under normal and a variety of pathological conditions.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Michailova, Anushka and Saucerman, Jeffrey and Belik, Mary Ellen and McCulloch, Andrew D}, biburl = {https://www.bibsonomy.org/bibtex/224a06278d96258b34abbc100237ad97a/hake}, description = {The whole bibliography file I use.}, doi = {10.1529/biophysj.104.046284}, file = {Mich_2005_2234.pdf:Mich_2005_2234.pdf:PDF}, institution = {U CA, San Diego}, interhash = {88c190d38cc1768a3be25afc2ac0cb31}, intrahash = {24a06278d96258b34abbc100237ad97a}, journal = {Biophys J}, keywords = {Adenosine Calcium Cardiac, Cardiovascular; Cells, Channel Channels, Computer Contraction, Cultured; Diphosphate, Gating, Homeostasis, Ion L-Type, Magnesium, Models, Myocardial Myocytes, Potassium Signal Signaling, Simulation; Transduction, Triphosphate, metabolism; physiology physiology;}, month = Mar, number = 3, pages = {2234--2249}, pdf = {Mich_2005_2234.pdf}, pii = {biophysj.104.046284}, pmid = {15738467}, timestamp = {2009-06-03T11:21:23.000+0200}, title = {Modeling regulation of cardiac KATP and L-type Ca2+ currents by ATP, ADP, and Mg2+.}, url = {http://dx.doi.org/10.1529/biophysj.104.046284}, volume = 88, year = 2005 }