The mitochondrial inner membrane harbors three protein translocases. Presequence translocase and carrier translocase are essential for importing nuclear-encoded proteins. The oxidase assembly (OXA) translocase is required for exporting mitochondrial-encoded proteins; however, different views exist about its relevance for nuclear-encoded proteins. We report that OXA plays a dual role in the biogenesis of nuclear-encoded mitochondrial proteins. First, a systematic analysis of OXA-deficient mitochondria led to~an unexpected expansion of the spectrum of OXA substrates imported via the presequence pathway. Second, biogenesis of numerous metabolite carriers depends on OXA, although they are not imported by~the presequence pathway. We show that OXA is crucial for the biogenesis of the Tim18-Sdh3 module of the carrier translocase. The export translocase OXA is thus required for the import of metabolite carriers by promoting assembly of the carrier translocase. We conclude that OXA is of central importance for the biogenesis of the mitochondrial inner membrane.
%0 Journal Article
%1 stillerMitochondrialOXATranslocase2016a
%A Stiller, Sebastian B.
%A Höpker, Jan
%A Oeljeklaus, Silke
%A Schütze, Conny
%A Schrempp, Sandra G.
%A Vent-Schmidt, Jens
%A Horvath, Susanne E.
%A Frazier, Ann E.
%A Gebert, Natalia
%A van der Laan, Martin
%A Bohnert, Maria
%A Warscheid, Bettina
%A Pfanner, Nikolaus
%A Wiedemann, Nils
%C United States
%D 2016
%J Cell metabolism
%K Cell Complex Dehydrogenase/metabolism,to_read IV/*metabolism,Membrane Membranes/*metabolism,Mitochondrial Nucleus/metabolism,Electron Proteins/*metabolism,Mitochondria/*enzymology,Mitochondrial Proteins/*metabolism,Mutation/genetics,Nuclear Proteins/*metabolism,Saccharomyces Proteins/metabolism,Saccharomyces Transport cerevisiae cerevisiae/*enzymology,Succinate
%N 5
%P 901--908
%R 10.1016/j.cmet.2016.04.005
%T Mitochondrial OXA Translocase Plays a Major Role in Biogenesis of Inner-Membrane Proteins.
%V 23
%X The mitochondrial inner membrane harbors three protein translocases. Presequence translocase and carrier translocase are essential for importing nuclear-encoded proteins. The oxidase assembly (OXA) translocase is required for exporting mitochondrial-encoded proteins; however, different views exist about its relevance for nuclear-encoded proteins. We report that OXA plays a dual role in the biogenesis of nuclear-encoded mitochondrial proteins. First, a systematic analysis of OXA-deficient mitochondria led to~an unexpected expansion of the spectrum of OXA substrates imported via the presequence pathway. Second, biogenesis of numerous metabolite carriers depends on OXA, although they are not imported by~the presequence pathway. We show that OXA is crucial for the biogenesis of the Tim18-Sdh3 module of the carrier translocase. The export translocase OXA is thus required for the import of metabolite carriers by promoting assembly of the carrier translocase. We conclude that OXA is of central importance for the biogenesis of the mitochondrial inner membrane.
@article{stillerMitochondrialOXATranslocase2016a,
abstract = {The mitochondrial inner membrane harbors three protein translocases. Presequence translocase and carrier translocase are essential for importing nuclear-encoded proteins. The oxidase assembly (OXA) translocase is required for exporting mitochondrial-encoded proteins; however, different views exist about its relevance for nuclear-encoded proteins. We report that OXA plays a dual role in the biogenesis of nuclear-encoded mitochondrial proteins. First, a systematic analysis of OXA-deficient mitochondria led to~an unexpected expansion of the spectrum of OXA substrates imported via the presequence pathway. Second, biogenesis of numerous metabolite carriers depends on OXA, although they are not imported by~the presequence pathway. We show that OXA is crucial for the biogenesis of the Tim18-Sdh3 module of the carrier translocase. The export translocase OXA is thus required for the import of metabolite carriers by promoting assembly of the carrier translocase. We conclude that OXA is of central importance for the biogenesis of the mitochondrial inner membrane.},
added-at = {2024-05-17T13:01:35.000+0200},
address = {United States},
author = {Stiller, Sebastian B. and H{\"o}pker, Jan and Oeljeklaus, Silke and Sch{\"u}tze, Conny and Schrempp, Sandra G. and {Vent-Schmidt}, Jens and Horvath, Susanne E. and Frazier, Ann E. and Gebert, Natalia and {van der Laan}, Martin and Bohnert, Maria and Warscheid, Bettina and Pfanner, Nikolaus and Wiedemann, Nils},
biburl = {https://www.bibsonomy.org/bibtex/228105da9b3eabdeebbb86b96206ace08/warscheidlab},
copyright = {Copyright {\copyright} 2016 The Authors. Published by Elsevier Inc. All rights reserved.},
doi = {10.1016/j.cmet.2016.04.005},
interhash = {d0151360ebd542dbf458535eca661054},
intrahash = {28105da9b3eabdeebbb86b96206ace08},
issn = {1932-7420 1550-4131},
journal = {Cell metabolism},
keywords = {Cell Complex Dehydrogenase/metabolism,to_read IV/*metabolism,Membrane Membranes/*metabolism,Mitochondrial Nucleus/metabolism,Electron Proteins/*metabolism,Mitochondria/*enzymology,Mitochondrial Proteins/*metabolism,Mutation/genetics,Nuclear Proteins/*metabolism,Saccharomyces Proteins/metabolism,Saccharomyces Transport cerevisiae cerevisiae/*enzymology,Succinate},
langid = {english},
month = may,
number = 5,
pages = {901--908},
pmcid = {PMC4873616},
pmid = {27166948},
timestamp = {2024-05-17T13:01:35.000+0200},
title = {Mitochondrial {{OXA Translocase Plays}} a {{Major Role}} in {{Biogenesis}} of {{Inner-Membrane Proteins}}.},
volume = 23,
year = 2016
}