%0 Journal Article %1 Winterflood2015 %A Winterflood, Christian M. %A Platonova, Evgenia %A Albrecht, David %A Ewers, Helge %D 2015 %I Cell Press %J Biophysical Journal %K 3D imaging microscopy multicolor multiplexing spectral_demixing superresolution %N 1 %P 3--6 %R 10.1016/j.bpj.2015.05.026 %T Dual-Color 3D Superresolution Microscopy by Combined Spectral-Demixing and Biplane Imaging %U https://www.sciencedirect.com/science/article/pii/S000634951500538X %V 109 %X Abstract Multicolor three-dimensional (3D) superresolution techniques allow important insight into the relative organization of cellular structures. While a number of innovative solutions have emerged, multicolor 3D techniques still face significant technical challenges. In this Letter we provide a straightforward approach to single-molecule localization microscopy imaging in three dimensions and two colors. We combine biplane imaging and spectral-demixing, which eliminates a number of problems, including color cross-talk, chromatic aberration effects, and problems with color registration. We present 3D dual-color images of nanoscopic structures in hippocampal neurons with a 3D compound resolution routinely achieved only in a single color.

@article{Winterflood2015, abstract = {Abstract Multicolor three-dimensional (3D) superresolution techniques allow important insight into the relative organization of cellular structures. While a number of innovative solutions have emerged, multicolor 3D techniques still face significant technical challenges. In this Letter we provide a straightforward approach to single-molecule localization microscopy imaging in three dimensions and two colors. We combine biplane imaging and spectral-demixing, which eliminates a number of problems, including color cross-talk, chromatic aberration effects, and problems with color registration. We present 3D dual-color images of nanoscopic structures in hippocampal neurons with a 3D compound resolution routinely achieved only in a single color.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Winterflood, Christian M. and Platonova, Evgenia and Albrecht, David and Ewers, Helge}, biburl = {https://www.bibsonomy.org/bibtex/22aba3221a677efe9d294182d9f3ff16e/kfriedl}, doi = {10.1016/j.bpj.2015.05.026}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Winterflood et al. - 2015 - Dual-Color 3D Superresolution Microscopy by Combined Spectral-Demixing and Biplane Imaging.pdf:pdf}, interhash = {af5177cbfebd0edde0f38078fa36f5fc}, intrahash = {2aba3221a677efe9d294182d9f3ff16e}, issn = {15420086}, journal = {Biophysical Journal}, keywords = {3D imaging microscopy multicolor multiplexing spectral_demixing superresolution}, month = jul, number = 1, pages = {3--6}, pmid = {26153696}, publisher = {Cell Press}, timestamp = {2020-04-06T15:19:11.000+0200}, title = {{Dual-Color 3D Superresolution Microscopy by Combined Spectral-Demixing and Biplane Imaging}}, url = {https://www.sciencedirect.com/science/article/pii/S000634951500538X}, volume = 109, year = 2015 }