%0 Journal Article %1 killoran_rapid_2006 %A Killoran, Patrick B. %A O'Connell, Janice %A Mothershed, Elizabeth A. %A Probert, Will S. %D 2006 %J Journal of Microbiological Methods %K Meningococcal Molecular Tandem disease, repeats subtyping, %N 2 %P 330--338 %R 10.1016/j.mimet.2006.04.004 %T Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis %U http://www.sciencedirect.com/science/article/B6T30-4K428GC-2/2/7759e2053e6abc09da28312234fa5ff1 %V 67 %X Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis (MLVA) for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis (PFGE). For MLVA, a multiplex PCR assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. MLVA was highly reproducible and provided results within 6 h. Overall, the discriminatory power of MLVA was equivalent to that of PFGE. The utilization of MLVA for subtyping N. meningitidis isolates provides a rapid and safer alternative to PFGE for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis.

@article{killoran_rapid_2006, abstract = {Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis {(MLVA)} for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis {(PFGE).} For {MLVA,} a multiplex {PCR} assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. {MLVA} was highly reproducible and provided results within 6 h. Overall, the discriminatory power of {MLVA} was equivalent to that of {PFGE.} The utilization of {MLVA} for subtyping N. meningitidis isolates provides a rapid and safer alternative to {PFGE} for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Killoran, Patrick B. and {O'Connell}, Janice and Mothershed, Elizabeth A. and Probert, Will S.}, biburl = {https://www.bibsonomy.org/bibtex/22bf70e88afec4084bdaf31006ea43fa5/jelias}, doi = {10.1016/j.mimet.2006.04.004}, interhash = {683ffd80d7d9823290f0c2611765ccfa}, intrahash = {2bf70e88afec4084bdaf31006ea43fa5}, issn = {0167-7012}, journal = {Journal of Microbiological Methods}, keywords = {Meningococcal Molecular Tandem disease, repeats subtyping,}, month = nov, number = 2, pages = {330--338}, timestamp = {2011-03-11T10:06:40.000+0100}, title = {Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis}, url = {http://www.sciencedirect.com/science/article/B6T30-4K428GC-2/2/7759e2053e6abc09da28312234fa5ff1}, volume = 67, year = 2006 }