A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B. We determined values for apolipoproteins A-I and B in frozen and lyophilized serum pools, using different immunochemical approaches. We found little or no differences in apolipoprotein A-I values between frozen and lyophilized pools as determined by the different methods. In contrast, values for apolipoprotein B in lyophilized samples were consistently lower than those obtained for frozen samples. After adjusting for the effect of dilution due to reconstitution, the difference in the apolipoprotein B values for lyophilized as compared with frozen samples ranged from -26% to 4%, depending upon the assay method. Evidently, serum pools in lyophilized from are not a suitable matrix for reference materials for apolipoprotein B measurements but can be used for apolipoprotein A-I measurements.
%0 Journal Article
%1 citeulike:566864
%A Marcovina, S. M.
%A Adolphson, J. L.
%A Parlavecchia, M.
%A Albers, J. J.
%C Northwest Lipid Research Center, Harborview Medical Center, Seattle, WA 98104-2499.
%D 1990
%J Clin Chem
%K methodology immunoassay apob
%N 2
%P 366--369
%T Effects of lyophilization of serum on the measurement of apolipoproteins A-I and B.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2105863
%V 36
%X A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B. We determined values for apolipoproteins A-I and B in frozen and lyophilized serum pools, using different immunochemical approaches. We found little or no differences in apolipoprotein A-I values between frozen and lyophilized pools as determined by the different methods. In contrast, values for apolipoprotein B in lyophilized samples were consistently lower than those obtained for frozen samples. After adjusting for the effect of dilution due to reconstitution, the difference in the apolipoprotein B values for lyophilized as compared with frozen samples ranged from -26% to 4%, depending upon the assay method. Evidently, serum pools in lyophilized from are not a suitable matrix for reference materials for apolipoprotein B measurements but can be used for apolipoprotein A-I measurements.
@article{citeulike:566864,
abstract = {A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B. We determined values for apolipoproteins A-I and B in frozen and lyophilized serum pools, using different immunochemical approaches. We found little or no differences in apolipoprotein A-I values between frozen and lyophilized pools as determined by the different methods. In contrast, values for apolipoprotein B in lyophilized samples were consistently lower than those obtained for frozen samples. After adjusting for the effect of dilution due to reconstitution, the difference in the apolipoprotein B values for lyophilized as compared with frozen samples ranged from -26% to 4%, depending upon the assay method. Evidently, serum pools in lyophilized from are not a suitable matrix for reference materials for apolipoprotein B measurements but can be used for apolipoprotein A-I measurements.},
added-at = {2006-07-07T01:10:50.000+0200},
address = {Northwest Lipid Research Center, Harborview Medical Center, Seattle, WA 98104-2499.},
author = {Marcovina, S. M. and Adolphson, J. L. and Parlavecchia, M. and Albers, J. J.},
biburl = {https://www.bibsonomy.org/bibtex/22c5b4cd125fe6db9d53102db776d8543/biblio24},
citeulike-article-id = {566864},
interhash = {98418f85bf365c3a458c9fa7ce2a46ae},
intrahash = {2c5b4cd125fe6db9d53102db776d8543},
issn = {0009-9147},
journal = {Clin Chem},
keywords = {methodology immunoassay apob},
month = {February},
number = 2,
pages = {366--369},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Effects of lyophilization of serum on the measurement of apolipoproteins A-I and B.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=2105863},
volume = 36,
year = 1990
}