%0 Journal Article %1 dolezalBTG2PRMT1ModuleLimits2017 %A Dolezal, Elmar %A Infantino, Simona %A Drepper, Friedel %A Börsig, Theresa %A Singh, Aparajita %A Wossning, Thomas %A Fiala, Gina J. %A Minguet, Susana %A Warscheid, Bettina %A Tarlinton, David M. %A Jumaa, Hassan %A Medgyesi, David %A Reth, Michael %C United States %D 2017 %J Nature immunology %K 4/*metabolism,DNA-Binding Animals,Cell B-Lymphocyte/genetics,Genes B-Lymphoid/cytology/*metabolism,Protein-Arginine Cells Chain Chains/genetics,Lymphopoiesis/*genetics,Mass Checkpoints,Cell Cycle Cytometry,Gene D3/*metabolism,Cyclin-Dependent Differentiation/genetics,Cell Interfering,to_read,Tumor Kinase Knockdown Light N-Methyltransferases/*genetics/metabolism,Real-Time Polymerase Proliferation/*genetics,Cyclin Proteins/*genetics/metabolism Proteins/*genetics/metabolism,Immunoglobulin Proteins/genetics/metabolism,Flow Proteins/genetics/metabolism,Immediate-Early Reaction,RNA Rearrangement Small Spectrometry,Mice,Precursor Suppressor Techniques,Gene abl/genetics,Homeodomain %N 8 %P 911--920 %R 10.1038/ni.3774 %T The BTG2-PRMT1 Module Limits Pre-B Cell Expansion by Regulating the CDK4-Cyclin-D3 Complex. %V 18 %X Developing pre-B cells in the bone marrow alternate between proliferation and differentiation phases. We found that protein arginine methyl transferase 1 (PRMT1) and B cell translocation gene 2 (BTG2) are critical components of the pre-B cell differentiation program. The BTG2-PRMT1 module induced a cell-cycle arrest of pre-B cells that was accompanied by re-expression of Rag1 and Rag2 and the onset of immunoglobulin light chain gene rearrangements. We found that PRMT1 methylated cyclin-dependent kinase 4 (CDK4), thereby preventing the formation of a CDK4-Cyclin-D3 complex and cell cycle progression. Moreover, BTG2 in concert with PRMT1 efficiently blocked the proliferation of BCR-ABL1-transformed pre-B cells in vitro and in vivo. Our results identify a key molecular mechanism by which the BTG2-PRMT1 module regulates pre-B cell differentiation and inhibits pre-B cell leukemogenesis.

@article{dolezalBTG2PRMT1ModuleLimits2017, abstract = {Developing pre-B cells in the bone marrow alternate between proliferation and differentiation phases. We found that protein arginine methyl transferase 1 (PRMT1) and B cell translocation gene 2 (BTG2) are critical components of the pre-B cell differentiation program. The BTG2-PRMT1 module induced a cell-cycle arrest of pre-B cells that was accompanied by re-expression of Rag1 and Rag2 and the onset of immunoglobulin light chain gene rearrangements. We found that PRMT1 methylated cyclin-dependent kinase 4 (CDK4), thereby preventing the formation of a CDK4-Cyclin-D3 complex and cell cycle progression. Moreover, BTG2 in concert with PRMT1 efficiently blocked the proliferation of BCR-ABL1-transformed pre-B cells in vitro and in vivo. Our results identify a key molecular mechanism by which the BTG2-PRMT1 module regulates pre-B cell differentiation and inhibits pre-B cell leukemogenesis.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {United States}, author = {Dolezal, Elmar and Infantino, Simona and Drepper, Friedel and B{\"o}rsig, Theresa and Singh, Aparajita and Wossning, Thomas and Fiala, Gina J. and Minguet, Susana and Warscheid, Bettina and Tarlinton, David M. and Jumaa, Hassan and Medgyesi, David and Reth, Michael}, biburl = {https://www.bibsonomy.org/bibtex/28d4fd7734586ae51d9035826855d5c43/warscheidlab}, doi = {10.1038/ni.3774}, interhash = {23434109b8e78b4af71a11d970a33f55}, intrahash = {8d4fd7734586ae51d9035826855d5c43}, issn = {1529-2916 1529-2908}, journal = {Nature immunology}, keywords = {4/*metabolism,DNA-Binding Animals,Cell B-Lymphocyte/genetics,Genes B-Lymphoid/cytology/*metabolism,Protein-Arginine Cells Chain Chains/genetics,Lymphopoiesis/*genetics,Mass Checkpoints,Cell Cycle Cytometry,Gene D3/*metabolism,Cyclin-Dependent Differentiation/genetics,Cell Interfering,to_read,Tumor Kinase Knockdown Light N-Methyltransferases/*genetics/metabolism,Real-Time Polymerase Proliferation/*genetics,Cyclin Proteins/*genetics/metabolism Proteins/*genetics/metabolism,Immunoglobulin Proteins/genetics/metabolism,Flow Proteins/genetics/metabolism,Immediate-Early Reaction,RNA Rearrangement Small Spectrometry,Mice,Precursor Suppressor Techniques,Gene abl/genetics,Homeodomain}, langid = {english}, month = aug, number = 8, pages = {911--920}, pmid = {28628091}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {The {{BTG2-PRMT1}} Module Limits Pre-{{B}} Cell Expansion by Regulating the {{CDK4-Cyclin-D3}} Complex.}, volume = 18, year = 2017 }