%0 Journal Article %1 Lope_1995_1042 %A L�pez-L�pez, J. R. %A Shacklock, P. S. %A Balke, C. W. %A Wier, W. G. %D 1995 %J Science %K 7754383 Animals, Calcium Calcium, Channel Channels, Confocal, Electric Gating, Gov't, Guinea In Ion Membrane Microscopy, Myocardium, Non-U.S., P.H.S., Patch-Clamp Pigs, Potentials, Probability, Research Reticulum, Sarcoplasmic Stimulation, Support, Techniques, U.S. Verapamil, Vitro, %N 5213 %P 1042--1045 %T Local calcium transients triggered by single L-type calcium channel currents in cardiac cells. %V 268 %X Excitation-contraction coupling was studied in mammalian cardiac cells in which the opening probability of L-type calcium (Ca$^2+$) channels was reduced. Confocal microscopy during voltage-clamp depolarization revealed distinct local transients in the concentration of intracellular calcium ions (Ca$^2+$i). When voltage was varied, the latency to occurrence and the relative probability of occurrence of local Ca$^2+$i transients varied as predicted if Ca$^2+$ release from the sarcoplasmic reticulum (SR) was linked tightly to Ca$^2+$ flux through L-type Ca$^2+$ channels but not to that through the Na-Ca exchanger or to average Ca$^2+$i. Voltage had no effect on the amplitude of local Ca$^2+$i transients. Thus, the most efficacious "Ca$^2+$ signal" for activating Ca$^2+$ release from the SR may be a transient microdomain of high Ca$^2+$i beneath an individual, open L-type Ca$^2+$ channel.

@article{Lope_1995_1042, abstract = {Excitation-contraction coupling was studied in mammalian cardiac cells in which the opening probability of L-type calcium ({C}a$^{2+}$) channels was reduced. Confocal microscopy during voltage-clamp depolarization revealed distinct local transients in the concentration of intracellular calcium ions ([{C}a$^{2+}$]i). When voltage was varied, the latency to occurrence and the relative probability of occurrence of local [{C}a$^{2+}$]i transients varied as predicted if {C}a$^{2+}$ release from the sarcoplasmic reticulum (SR) was linked tightly to {C}a$^{2+}$ flux through L-type {C}a$^{2+}$ channels but not to that through the Na-Ca exchanger or to average [{C}a$^{2+}$]i. Voltage had no effect on the amplitude of local [{C}a$^{2+}$]i transients. Thus, the most efficacious "{C}a$^{2+}$ signal" for activating {C}a$^{2+}$ release from the SR may be a transient microdomain of high [{C}a$^{2+}$]i beneath an individual, open L-type {C}a$^{2+}$ channel.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {L�pez-L�pez, J. R. and Shacklock, P. S. and Balke, C. W. and Wier, W. G.}, biburl = {https://www.bibsonomy.org/bibtex/23a283cf36faac93ebce1528e44acb15a/hake}, description = {The whole bibliography file I use.}, file = {Lope_1995_1042.pdf:Lope_1995_1042.pdf:PDF}, interhash = {94389751ff7b84d8c1f2e72a8509a89b}, intrahash = {3a283cf36faac93ebce1528e44acb15a}, journal = {Science}, key = 94, keywords = {7754383 Animals, Calcium Calcium, Channel Channels, Confocal, Electric Gating, Gov't, Guinea In Ion Membrane Microscopy, Myocardium, Non-U.S., P.H.S., Patch-Clamp Pigs, Potentials, Probability, Research Reticulum, Sarcoplasmic Stimulation, Support, Techniques, U.S. Verapamil, Vitro,}, month = May, number = 5213, pages = {1042--1045}, pmid = {7754383}, timestamp = {2009-06-03T11:21:21.000+0200}, title = {Local calcium transients triggered by single L-type calcium channel currents in cardiac cells.}, volume = 268, year = 1995 }