%0 Journal Article %1 RN1052 %A Deeken, R. %A Saupe, S. %A Klinkenberg, J. %A Riedel, M. %A Leide, J. %A Hedrich, R. %A Mueller, T. D. %D 2016 %J Plant Physiology %K expression myOwn tissue-specific %N 3 %P 1911-1927 %R 10.1104/pp.16.01486 %T The Nonspecific Lipid Transfer Protein AtLtpI-4 Is Involved in Suberin Formation of Crown Galls %U /brokenurl#<Go to ISI>://WOS:000391172300041 %V 172 %X Nonspecific lipid transfer proteins reversibly bind different types of lipid molecules in a hydrophobic cavity. They facilitate phospholipid transfer between membranes in vitro, play a role in cuticle and possibly in suberin formation, and might be involved in plant pathogen defense signaling. This study focuses on the role of the lipid transfer protein AtLTPI-4 in crown gall development. Arabidopsis (Arabidopsis thaliana) crown gall tumors, which develop upon infection with the virulent Agrobacterium tumefaciens strain C-58, highly expressed AtLTPI-4. Crown galls of the atltpI-4 loss-of-function mutant were much smaller compared with those of wild-type plants. The gene expression pattern and localization of the protein to the plasma membrane pointed to a function of AtLTPI-4 in cell wall suberization. Since Arabidopsis crown galls are covered by a suberin-containing periderm instead of a cuticle, we analyzed the suberin composition of crown galls and found a reduction in the amounts of long-chain fatty acids (C-18:0) in the atltpI-4 mutant. To demonstrate the impact of AtLtpI-4 on extracellular lipid composition, we expressed the protein in Arabidopsis epidermis cells. This led to a significant increase in the very-long-chain fatty acids C-24 and C-26 in the cuticular wax fraction. Homology modeling and lipid-protein-overlay assays showed that AtLtpI-4 protein can bind these very-long-chain fatty acids. Thus, AtLtpI-4 protein may facilitate the transfer of long-chain as well as very-long-chain fatty acids into the apoplast, depending on the cell type in which it is expressed. In crown galls, which endogenously express AtLtpI-4, it is involved in suberin formation.

@article{RN1052, abstract = {Nonspecific lipid transfer proteins reversibly bind different types of lipid molecules in a hydrophobic cavity. They facilitate phospholipid transfer between membranes in vitro, play a role in cuticle and possibly in suberin formation, and might be involved in plant pathogen defense signaling. This study focuses on the role of the lipid transfer protein AtLTPI-4 in crown gall development. Arabidopsis (Arabidopsis thaliana) crown gall tumors, which develop upon infection with the virulent Agrobacterium tumefaciens strain C-58, highly expressed AtLTPI-4. Crown galls of the atltpI-4 loss-of-function mutant were much smaller compared with those of wild-type plants. The gene expression pattern and localization of the protein to the plasma membrane pointed to a function of AtLTPI-4 in cell wall suberization. Since Arabidopsis crown galls are covered by a suberin-containing periderm instead of a cuticle, we analyzed the suberin composition of crown galls and found a reduction in the amounts of long-chain fatty acids (C-18:0) in the atltpI-4 mutant. To demonstrate the impact of AtLtpI-4 on extracellular lipid composition, we expressed the protein in Arabidopsis epidermis cells. This led to a significant increase in the very-long-chain fatty acids C-24 and C-26 in the cuticular wax fraction. Homology modeling and lipid-protein-overlay assays showed that AtLtpI-4 protein can bind these very-long-chain fatty acids. Thus, AtLtpI-4 protein may facilitate the transfer of long-chain as well as very-long-chain fatty acids into the apoplast, depending on the cell type in which it is expressed. In crown galls, which endogenously express AtLtpI-4, it is involved in suberin formation.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Deeken, R. and Saupe, S. and Klinkenberg, J. and Riedel, M. and Leide, J. and Hedrich, R. and Mueller, T. D.}, biburl = {https://www.bibsonomy.org/bibtex/23b71959943cff3c8f27da5e9bffe9069/rainerhedrich_2}, doi = {10.1104/pp.16.01486}, interhash = {9d019562e1fb61f9e4a7ef364c22b8dd}, intrahash = {3b71959943cff3c8f27da5e9bffe9069}, issn = {0032-0889}, journal = {Plant Physiology}, keywords = {expression myOwn tissue-specific}, note = {Eg6qz Times Cited:50 Cited References Count:95}, number = 3, pages = {1911-1927}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {The Nonspecific Lipid Transfer Protein AtLtpI-4 Is Involved in Suberin Formation of Crown Galls}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000391172300041}, volume = 172, year = 2016 }