In this novel enzyme-tagged immuoelectrochemical assay, disposable carbon felt discs serve both as electrodes and as the heterogeneous solid phase. Antibodies are immobilized on the carbon felt via a diaminoalkane-biotin-avidin-biotin bridge. Alkaline phosphatase is used as a label. Bound antibodies are monitored by following the electro-oxidation of aminophenol, produced enzymatically from p-amino-phenyl phosphate by the immobilized alkaline phosphatase at the electrode surface. A model system designed for determination of mouse IgG concentration yielded a calibration curve ranging from 10 pg/ml to 100 micrograms/ml. This assay can be performed rapidly and a single determination completed within 20 minutes. The system is useful also for rapid quantitation of a small number (approximately 80 organisms per ml) of bacteria.
%0 Journal Article
%1 citeulike:693520
%A Hadas, E.
%A Soussan, L.
%A Rosen-Margalit, I.
%A Farkash, A.
%A Rishpon, J.
%C Department of Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Israel.
%D 1992
%J J Immunoassay
%K amperometry a-p immunoassay electrochemistry heterogeneous immunoelectrode
%N 2
%P 231--252
%T A rapid and sensitive heterogeneous immunoelectrochemical assay using disposable electrodes.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1430241
%V 13
%X In this novel enzyme-tagged immuoelectrochemical assay, disposable carbon felt discs serve both as electrodes and as the heterogeneous solid phase. Antibodies are immobilized on the carbon felt via a diaminoalkane-biotin-avidin-biotin bridge. Alkaline phosphatase is used as a label. Bound antibodies are monitored by following the electro-oxidation of aminophenol, produced enzymatically from p-amino-phenyl phosphate by the immobilized alkaline phosphatase at the electrode surface. A model system designed for determination of mouse IgG concentration yielded a calibration curve ranging from 10 pg/ml to 100 micrograms/ml. This assay can be performed rapidly and a single determination completed within 20 minutes. The system is useful also for rapid quantitation of a small number (approximately 80 organisms per ml) of bacteria.
@article{citeulike:693520,
abstract = {In this novel enzyme-tagged immuoelectrochemical assay, disposable carbon felt discs serve both as electrodes and as the heterogeneous solid phase. Antibodies are immobilized on the carbon felt via a diaminoalkane-biotin-avidin-biotin bridge. Alkaline phosphatase is used as a label. Bound antibodies are monitored by following the electro-oxidation of aminophenol, produced enzymatically from p-amino-phenyl phosphate by the immobilized alkaline phosphatase at the electrode surface. A model system designed for determination of mouse IgG concentration yielded a calibration curve ranging from 10 pg/ml to 100 micrograms/ml. This assay can be performed rapidly and a single determination completed within 20 minutes. The system is useful also for rapid quantitation of a small number (approximately 80 organisms per ml) of bacteria.},
added-at = {2006-07-07T01:10:50.000+0200},
address = {Department of Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Israel.},
author = {Hadas, E. and Soussan, L. and Rosen-Margalit, I. and Farkash, A. and Rishpon, J.},
biburl = {https://www.bibsonomy.org/bibtex/251eafae920c4a4b40ff71fdddbbbde0d/biblio24},
citeulike-article-id = {693520},
interhash = {3fb769072dcf2047efefabd984afdff8},
intrahash = {51eafae920c4a4b40ff71fdddbbbde0d},
issn = {0197-1522},
journal = {J Immunoassay},
keywords = {amperometry a-p immunoassay electrochemistry heterogeneous immunoelectrode},
number = 2,
pages = {231--252},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {A rapid and sensitive heterogeneous immunoelectrochemical assay using disposable electrodes.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=1430241},
volume = 13,
year = 1992
}