%0 Journal Article %1 fish2017methylation %A Fish, Trevor J. %A Benninghoff, Abby D. %D 2017 %J Food and Chemical Toxicology %K 6 cancer epigenetics methylation %P 1-11 %R 10.1016/j.fct.2017.04.047 %T DNA methylation in lung tissue of mouse offspring exposed in utero to polycyclic aromatic hydrocarbons %U http://www.sciencedirect.com/science/article/pii/S0278691517302247 %V xxx %X Polycyclic aromatic hydrocarbons (PAHs) comprise an important class of environmental pollutants that are known to cause lung cancer in animals and are suspected lung carcinogens in humans. Moreover, evidence from cell-based studies points to PAHs as modulators of the epigenome. The objective of this work was to assess patterns of genome-wide DNA methylation in lung tissues of adult offspring initiated in utero with the transplacental PAH carcinogens dibenzo def,pchrysene (DBC) or benzo apyrene (BaP). Genome-wide methylation patterns for normal (not exposed), normal adjacent and lung tumor tissues obtained from adult offspring were determined using methylated DNA immunoprecipitation (MeDIP) with the NimbleGen mouse DNA methylation CpG island array. Lung tumor incidence in 45-week old mice initiated with BaP was 32%, much lower than that of the DBC-exposed offspring at 96%. Also, male offspring appeared more susceptible to BaP as compared to females. Distinct patterns of DNA methylation were associated with non-exposed, normal adjacent and adenocarcinoma lung tissues, as determined by principal components, hierarchical clustering and gene ontology analyses. From these methylation profiles, a set of genes of interest was identified that includes potential important targets for epigenetic modification during the process of lung tumorigenesis in animals exposed to environmental PAHs.

@article{fish2017methylation, abstract = {Polycyclic aromatic hydrocarbons (PAHs) comprise an important class of environmental pollutants that are known to cause lung cancer in animals and are suspected lung carcinogens in humans. Moreover, evidence from cell-based studies points to PAHs as modulators of the epigenome. The objective of this work was to assess patterns of genome-wide DNA methylation in lung tissues of adult offspring initiated in utero with the transplacental PAH carcinogens dibenzo [def,p]chrysene (DBC) or benzo [a]pyrene (BaP). Genome-wide methylation patterns for normal (not exposed), normal adjacent and lung tumor tissues obtained from adult offspring were determined using methylated DNA immunoprecipitation (MeDIP) with the NimbleGen mouse DNA methylation CpG island array. Lung tumor incidence in 45-week old mice initiated with BaP was 32%, much lower than that of the DBC-exposed offspring at 96%. Also, male offspring appeared more susceptible to BaP as compared to females. Distinct patterns of DNA methylation were associated with non-exposed, normal adjacent and adenocarcinoma lung tissues, as determined by principal components, hierarchical clustering and gene ontology analyses. From these methylation profiles, a set of genes of interest was identified that includes potential important targets for epigenetic modification during the process of lung tumorigenesis in animals exposed to environmental PAHs.}, added-at = {2017-10-12T20:07:38.000+0200}, author = {Fish, Trevor J. and Benninghoff, Abby D.}, biburl = {https://www.bibsonomy.org/bibtex/2534fac0cdd51550fcb6b6db52bd19115/artheibault}, description = {This article discusses the impact of prenatal polycyclic aromatic hydrocarbon exposure on methylation patterns in mice. Of particular interest is the test subjects' vulnerability to tumors in the lungs. Overall the article demonstrates a link between polycycylic aromatic hydrocarbon exposure and DNA methylation.}, doi = {10.1016/j.fct.2017.04.047}, interhash = {8e08687fc4b3464aae539461739d9ebf}, intrahash = {534fac0cdd51550fcb6b6db52bd19115}, journal = {Food and Chemical Toxicology}, keywords = {6 cancer epigenetics methylation}, pages = {1-11}, timestamp = {2017-10-26T14:43:32.000+0200}, title = {DNA methylation in lung tissue of mouse offspring exposed in utero to polycyclic aromatic hydrocarbons}, url = {http://www.sciencedirect.com/science/article/pii/S0278691517302247}, volume = {xxx}, year = 2017 }