%0 Journal Article %1 pournaras_hospital_2003 %A Pournaras, S %A Maniati, M %A Petinaki, E %A Tzouvelekis, L S %A Tsakris, A %A Legakis, N J %A Maniatis, A N %D 2003 %J The Journal of Antimicrobial Chemotherapy %K Agents, Bacterial, Cells, Clone Cross Disease Drug Humans, Infection, Infections, Outbreaks, Pseudomonas Resistance, Variation aeruginosa, {(Genetics)} {Anti-Bacterial} {beta-Lactamases,} %N 6 %P 1409--14 %R 12716773 %T Hospital outbreak of multiple clones of Pseudomonas aeruginosa carrying the unrelated metallo-beta-lactamase gene variants blaVIM-2 and blaVIM-4 %U http://www.ncbi.nlm.nih.gov/pubmed/12716773 %V 51 %X OBJECTIVES: The possible contribution of metallo-beta-lactamases in the frequent detection of carbapenem-resistant Pseudomonas aeruginosa isolates in a tertiary Greek hospital in Central Greece was investigated.Materials and methods: All carbapenem-resistant (imipenem- and/or meropenem-resistant) P. aeruginosa isolates recovered from separate patients during a 1 year period in the Clinical Microbiology Laboratory at the University Hospital of Thessaly, Larissa, Greece, were studied for metallo-beta-lactamases. They were tested by Etest MBL, PCR analysis and nucleotide sequencing. DNA fingerprints were obtained by pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA. RESULTS: A blaVIM gene was detected in 47 of the 53 (88.7\%) carbapenem-resistant P. aeruginosa isolates. PFGE grouped the blaVIM-positive isolates in six unrelated genotypes; one type included two subtypes. Nucleotide sequencing of the PCR amplicons of a randomly selected isolate from each one of the seven subtypes, detected the variant sequences blaVIM-2 in four and blaVIM-4 in three cases, respectively. They were carried as single gene cassettes or along with an aminoglycoside resistance gene (aacA29a) in class 1 integrons. CONCLUSIONS: These findings suggest that different strains of P. aeruginosa carrying unrelated metallo-beta-lactamase gene variants predominate in our hospital environment.

@article{pournaras_hospital_2003, abstract = {{OBJECTIVES:} The possible contribution of metallo-beta-lactamases in the frequent detection of carbapenem-resistant Pseudomonas aeruginosa isolates in a tertiary Greek hospital in Central Greece was {investigated.Materials} and methods: All carbapenem-resistant (imipenem- and/or meropenem-resistant) P. aeruginosa isolates recovered from separate patients during a 1 year period in the Clinical Microbiology Laboratory at the University Hospital of Thessaly, Larissa, Greece, were studied for metallo-beta-lactamases. They were tested by Etest {MBL,} {PCR} analysis and nucleotide sequencing. {DNA} fingerprints were obtained by pulsed-field gel electrophoresis {(PFGE)} of {XbaI-digested} chromosomal {DNA.} {RESULTS:} A {blaVIM} gene was detected in 47 of the 53 (88.7\%) carbapenem-resistant P. aeruginosa isolates. {PFGE} grouped the {blaVIM-positive} isolates in six unrelated genotypes; one type included two subtypes. Nucleotide sequencing of the {PCR} amplicons of a randomly selected isolate from each one of the seven subtypes, detected the variant sequences {blaVIM-2} in four and {blaVIM-4} in three cases, respectively. They were carried as single gene cassettes or along with an aminoglycoside resistance gene {(aacA29a)} in class 1 integrons. {CONCLUSIONS:} These findings suggest that different strains of P. aeruginosa carrying unrelated metallo-beta-lactamase gene variants predominate in our hospital environment.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Pournaras, S and Maniati, M and Petinaki, E and Tzouvelekis, L S and Tsakris, A and Legakis, N J and Maniatis, A N}, biburl = {https://www.bibsonomy.org/bibtex/256f86f9580e1baf2f020adac5168904e/jelias}, doi = {12716773}, interhash = {37c2bd85a56072a998e5f6ced3d537cc}, intrahash = {56f86f9580e1baf2f020adac5168904e}, issn = {0305-7453}, journal = {The Journal of Antimicrobial Chemotherapy}, keywords = {Agents, Bacterial, Cells, Clone Cross Disease Drug Humans, Infection, Infections, Outbreaks, Pseudomonas Resistance, Variation aeruginosa, {(Genetics)} {Anti-Bacterial} {beta-Lactamases,}}, month = jun, note = {{PMID:} 12716773}, number = 6, pages = {1409--14}, timestamp = {2011-03-11T10:06:11.000+0100}, title = {Hospital outbreak of multiple clones of Pseudomonas aeruginosa carrying the unrelated metallo-beta-lactamase gene variants {blaVIM-2} and {blaVIM-4}}, url = {http://www.ncbi.nlm.nih.gov/pubmed/12716773}, volume = 51, year = 2003 }