Optimizing the medium conditions for production
of tetracycline by solid state fermentation of
Streptomyces aureofaciens NCIM 2417 using
statistical experimental methods
Bioscience & Engineering: An International Journal (BIOEJ), 1 (1):
29 - 44 (July 2014)Abstract
Tetracycline belongs to one of the extracellular produced polyketide antibiotics having hydrophilic
properties. Statistical based optimization, Plackett–Burman design (PBD) and response surface
methodology (RSM) were occupied to screen and optimize the medium conditions for the
production of tetracycline from Streptomyces aureofaciens NCIM 2417, using solid state fermentation.
pineapple peel waste was used as both the curious solid support and carbon and nitrogen sources and
inorganic salts for the growth of Streptomyces aureofaciens NCIM 2417. Based on the positive
influence of the half normal plot obtained from PBD on tetracycline production, three medium
conditions – peanut meal, incubation period and soluble starch were screened. Central composite
design (CCD) was occupied using these three medium conditions at five levels, for advance
optimization, and the second order polynomial equation was derived, based on the experimental
data. Response surface methodology showed that the conditions of peanut meal (0.4%), incubation
period (day 2) and soluble starch (2%) were the optimal levels for maximal tetracycline production
(17.98 mg/g substrate) which were validated through experiments.