In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed thatJB interacts with the lipid bilayer at the level of the glycerol moieties.
%0 Journal Article
%1 RN193
%A Garcia-Beltran, O.
%A Cassels, B.K.
%A Mena, N.
%A Nunez, M.T.
%A Yanez, O.
%A Caballero, J.
%D 2014
%I 2013 Elsevier Ltd.
%J Tetrahedron Letters
%K bilayer, copper, coumarin cu2+-selectivity, derivative, design, dqcauchile dynamics, fluorescent lipid membranes molecular probe,
%N 4
%P 873-876
%R 10.1016/j.tetlet.2013.12.033
%T A Coumarinylaldoxime as a Specific Sensor for Cu2+ and Its Biological Application
%U /brokenurl#<Go to ISI>://WOS:000330604500023
%V 55
%X In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed thatJB interacts with the lipid bilayer at the level of the glycerol moieties.
@article{RN193,
abstract = {In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed thatJB interacts with the lipid bilayer at the level of the glycerol moieties. },
added-at = {2019-12-04T03:57:35.000+0100},
author = {Garcia-Beltran, O. and Cassels, B.K. and Mena, N. and Nunez, M.T. and Yanez, O. and Caballero, J.},
biburl = {https://www.bibsonomy.org/bibtex/27e21c75bcf0a0829309dc659bf6de19a/dqcauchile},
doi = {10.1016/j.tetlet.2013.12.033},
interhash = {bf006eacd2b04b6e7eb5133bcf0022d7},
intrahash = {7e21c75bcf0a0829309dc659bf6de19a},
issn = {0040-4039},
journal = {Tetrahedron Letters},
keywords = {bilayer, copper, coumarin cu2+-selectivity, derivative, design, dqcauchile dynamics, fluorescent lipid membranes molecular probe,},
number = 4,
pages = {873-876},
publisher = {2013 Elsevier Ltd.},
timestamp = {2019-12-04T03:58:17.000+0100},
title = {A Coumarinylaldoxime as a Specific Sensor for Cu2+ and Its Biological Application},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000330604500023},
volume = 55,
year = 2014
}