The development of a simple electrochemical immunoassay procedure for the field-based quantification of the herbicide 2,4-D in methanolic soil extracts is presented. The sensor utilizes a competitive immunoassay format incorporating an immobilized antigen complex at the surface of a disposable screen-printed working electrode element. The extent of glucose oxidase-labeled antibody binding to the antigen--electrode is determined amperometrically and is related to sample analyte concentration. The performance of the sensor is assessed in buffer, 30% methanol, and methanolic soil extracts. The device is capable of quantifying 2,4-D in all three matrixes at the low ppm level with coefficient of variation values of 6.2-33.6%. The causes of the variation observed in the sensor response in different soil matrixes are examined and improvements proposed. The sensor, tested in parallel with a commercial 2,4-D immunoassay test kit, yields comparable quantitative data and detection limits while exhibiting greater assay simplicity.
%0 Journal Article
%1 citeulike:471080
%A Kröger, S.
%A Setford, S. J.
%A Turner, A. P.
%C Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, U.K.
%D 1998
%J Anal Chem
%K amperometry competition immunoassay ag_detection electrochemistry
%N 23
%P 5047--5053
%R 10.1021/ac9805100
%T Immunosensor for 2,4-dichlorophenoxyacetic acid in aqueous/organic solvent soil extracts.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9852786
%V 70
%X The development of a simple electrochemical immunoassay procedure for the field-based quantification of the herbicide 2,4-D in methanolic soil extracts is presented. The sensor utilizes a competitive immunoassay format incorporating an immobilized antigen complex at the surface of a disposable screen-printed working electrode element. The extent of glucose oxidase-labeled antibody binding to the antigen--electrode is determined amperometrically and is related to sample analyte concentration. The performance of the sensor is assessed in buffer, 30% methanol, and methanolic soil extracts. The device is capable of quantifying 2,4-D in all three matrixes at the low ppm level with coefficient of variation values of 6.2-33.6%. The causes of the variation observed in the sensor response in different soil matrixes are examined and improvements proposed. The sensor, tested in parallel with a commercial 2,4-D immunoassay test kit, yields comparable quantitative data and detection limits while exhibiting greater assay simplicity.
@article{citeulike:471080,
abstract = {The development of a simple electrochemical immunoassay procedure for the field-based quantification of the herbicide 2,4-D in methanolic soil extracts is presented. The sensor utilizes a competitive immunoassay format incorporating an immobilized antigen complex at the surface of a disposable screen-printed working electrode element. The extent of glucose oxidase-labeled antibody binding to the antigen--electrode is determined amperometrically and is related to sample analyte concentration. The performance of the sensor is assessed in buffer, 30% methanol, and methanolic soil extracts. The device is capable of quantifying 2,4-D in all three matrixes at the low ppm level with coefficient of variation values of 6.2-33.6%. The causes of the variation observed in the sensor response in different soil matrixes are examined and improvements proposed. The sensor, tested in parallel with a commercial 2,4-D immunoassay test kit, yields comparable quantitative data and detection limits while exhibiting greater assay simplicity.},
added-at = {2006-07-07T01:10:50.000+0200},
address = {Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, U.K.},
author = {Kröger, S. and Setford, S. J. and Turner, A. P.},
biburl = {https://www.bibsonomy.org/bibtex/280305102d6b7f68e841c64bf5165fdce/biblio24},
citeulike-article-id = {471080},
doi = {10.1021/ac9805100},
interhash = {a38b6bfdcffdd410580dba23cbfe8366},
intrahash = {80305102d6b7f68e841c64bf5165fdce},
issn = {0003-2700},
journal = {Anal Chem},
keywords = {amperometry competition immunoassay ag_detection electrochemistry},
month = {December},
number = 23,
pages = {5047--5053},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Immunosensor for 2,4-dichlorophenoxyacetic acid in aqueous/organic solvent soil extracts.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9852786},
volume = 70,
year = 1998
}