A complement-mediated liposome immune lysis assay using entrapped calcein was developed for a plant toxin gelonin. Gelonin was covalently coupled to DPPE, and then adsorbed on to the surface of liposomes. Such antigen-bearing liposomes when incubated with anti-gelonin antibody in the presence of guinea pig complement undergo lysis. The detection range is from 3 ng to 60 ng. The method was used to monitor isolation of gelonin by affinity chromatography. It was observed that a minor peak in addition to the major one comes with gelonin, shared common epitopes/epitope with gelonin in immunological reaction. This was further confirmed by SDS-gel electrophoresis indicating the former being an isoform of gelonin. A comparative study of the immunocross-reactivity of ricin and ricin A chain with anti-gelonin antibody was carried out. It was found that while ricin A chain cross-reacted extensively with gelonin antibody and intact ricin elicited little or no cross-reactivity. It is suggested that the present LILA may be employed for the detection and quantitation of ricin A chain by this LILA method.
%0 Journal Article
%1 citeulike:469296
%A Paul, A.
%A Madan, S.
%A Vasandani, V. M.
%A Ghosh, P. C.
%A Bachhawat, B. K.
%C Department of Biochemistry, University of Delhi, India.
%D 1992
%J J Immunol Methods
%K l-i-l-a complement immunoassay homogeneous ag_detection
%N 1-2
%P 151--158
%T Liposome immune lysis assay (LILA) for gelonin.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1564325
%V 148
%X A complement-mediated liposome immune lysis assay using entrapped calcein was developed for a plant toxin gelonin. Gelonin was covalently coupled to DPPE, and then adsorbed on to the surface of liposomes. Such antigen-bearing liposomes when incubated with anti-gelonin antibody in the presence of guinea pig complement undergo lysis. The detection range is from 3 ng to 60 ng. The method was used to monitor isolation of gelonin by affinity chromatography. It was observed that a minor peak in addition to the major one comes with gelonin, shared common epitopes/epitope with gelonin in immunological reaction. This was further confirmed by SDS-gel electrophoresis indicating the former being an isoform of gelonin. A comparative study of the immunocross-reactivity of ricin and ricin A chain with anti-gelonin antibody was carried out. It was found that while ricin A chain cross-reacted extensively with gelonin antibody and intact ricin elicited little or no cross-reactivity. It is suggested that the present LILA may be employed for the detection and quantitation of ricin A chain by this LILA method.
@article{citeulike:469296,
abstract = {A complement-mediated liposome immune lysis assay using entrapped calcein was developed for a plant toxin gelonin. Gelonin was covalently coupled to DPPE, and then adsorbed on to the surface of liposomes. Such antigen-bearing liposomes when incubated with anti-gelonin antibody in the presence of guinea pig complement undergo lysis. The detection range is from 3 ng to 60 ng. The method was used to monitor isolation of gelonin by affinity chromatography. It was observed that a minor peak in addition to the major one comes with gelonin, shared common epitopes/epitope with gelonin in immunological reaction. This was further confirmed by SDS-gel electrophoresis indicating the former being an isoform of gelonin. A comparative study of the immunocross-reactivity of ricin and ricin A chain with anti-gelonin antibody was carried out. It was found that while ricin A chain cross-reacted extensively with gelonin antibody and intact ricin elicited little or no cross-reactivity. It is suggested that the present LILA may be employed for the detection and quantitation of ricin A chain by this LILA method.},
added-at = {2006-07-07T01:10:50.000+0200},
address = {Department of Biochemistry, University of Delhi, India.},
author = {Paul, A. and Madan, S. and Vasandani, V. M. and Ghosh, P. C. and Bachhawat, B. K.},
biburl = {https://www.bibsonomy.org/bibtex/2884673aef0542c7a25873a64d6372567/biblio24},
citeulike-article-id = {469296},
interhash = {e3f638ed7c396f88ee9e5b8e526984e6},
intrahash = {884673aef0542c7a25873a64d6372567},
issn = {0022-1759},
journal = {J Immunol Methods},
keywords = {l-i-l-a complement immunoassay homogeneous ag_detection},
month = {April},
number = {1-2},
pages = {151--158},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Liposome immune lysis assay (LILA) for gelonin.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=1564325},
volume = 148,
year = 1992
}