It is widely assumed that the key rate-limiting step in gene activation is the recruitment of RNA polymerase II (Pol II) to the core promoter. Although there are well-documented examples in which Pol II is recruited to a gene but stalls, a general role for Pol II stalling in development has not been established. We have carried out comprehensive Pol II chromatin immunoprecipitation microarray (ChIP-chip) assays in Drosophila embryos and identified three distinct Pol II binding behaviors: active (uniform binding across the entire transcription unit), no binding, and stalled (binding at the transcription start site). The notable feature of the approximately 10% genes that are stalled is that they are highly enriched for developmental control genes, which are either repressed or poised for activation during later stages of embryogenesis. We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development.
Description
RNA polymerase stalling at developmental control g... [Nat Genet. 2007] - PubMed result
%0 Journal Article
%1 zeitlinger2007polymerase
%A Zeitlinger, J
%A Stark, A
%A Kellis, M
%A Hong, J W
%A Nechaev, S
%A Adelman, K
%A Levine, M
%A Young, R A
%D 2007
%J Nat Genet
%K Drosophila pausing stochastic_transcription
%N 12
%P 1512-1516
%R 10.1038/ng.2007.26
%T RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
%U http://www.ncbi.nlm.nih.gov/pubmed/17994019
%V 39
%X It is widely assumed that the key rate-limiting step in gene activation is the recruitment of RNA polymerase II (Pol II) to the core promoter. Although there are well-documented examples in which Pol II is recruited to a gene but stalls, a general role for Pol II stalling in development has not been established. We have carried out comprehensive Pol II chromatin immunoprecipitation microarray (ChIP-chip) assays in Drosophila embryos and identified three distinct Pol II binding behaviors: active (uniform binding across the entire transcription unit), no binding, and stalled (binding at the transcription start site). The notable feature of the approximately 10% genes that are stalled is that they are highly enriched for developmental control genes, which are either repressed or poised for activation during later stages of embryogenesis. We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development.
@article{zeitlinger2007polymerase,
abstract = {It is widely assumed that the key rate-limiting step in gene activation is the recruitment of RNA polymerase II (Pol II) to the core promoter. Although there are well-documented examples in which Pol II is recruited to a gene but stalls, a general role for Pol II stalling in development has not been established. We have carried out comprehensive Pol II chromatin immunoprecipitation microarray (ChIP-chip) assays in Drosophila embryos and identified three distinct Pol II binding behaviors: active (uniform binding across the entire transcription unit), no binding, and stalled (binding at the transcription start site). The notable feature of the approximately 10% genes that are stalled is that they are highly enriched for developmental control genes, which are either repressed or poised for activation during later stages of embryogenesis. We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development.},
added-at = {2010-05-06T20:43:10.000+0200},
author = {Zeitlinger, J and Stark, A and Kellis, M and Hong, J W and Nechaev, S and Adelman, K and Levine, M and Young, R A},
biburl = {https://www.bibsonomy.org/bibtex/28a5268deaf38bc092912649f485ba3a2/peter.ralph},
description = {RNA polymerase stalling at developmental control g... [Nat Genet. 2007] - PubMed result},
doi = {10.1038/ng.2007.26},
interhash = {8d58f6971a2abb2d4ac9075bf7c9e6a0},
intrahash = {8a5268deaf38bc092912649f485ba3a2},
journal = {Nat Genet},
keywords = {Drosophila pausing stochastic_transcription},
month = dec,
number = 12,
pages = {1512-1516},
pmid = {17994019},
timestamp = {2010-05-06T20:43:10.000+0200},
title = {{RNA} polymerase stalling at developmental control genes in the {\it {D}rosophila melanogaster} embryo},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17994019},
volume = 39,
year = 2007
}