An improved growth medium for enhanced inoculum production of the plant growth-promoting fungus Serendipita indica
M. Osman, C. Stigloher, M. Mueller, и F. Waller. Plant Methods, (2020)Osman, Mohamed
Stigloher, Christian
Mueller, Martin J
Waller, Frank
eng
England
2020/03/20
Plant Methods. 2020 Mar 16;16:39. doi: 10.1186/s13007-020-00584-7. eCollection 2020..
DOI: 10.1186/s13007-020-00584-7
Аннотация
BACKGROUND: The plant endophytic fungus Serendipita indica colonizes roots of a wide range of plant species and can enhance growth and stress resistance of these plants. Due to its ease of axenic cultivation and its broad host plant range including the model plant Arabidopsis thaliana and numerous crop plants, it is widely used as a model fungus to study beneficial fungus-root interactions. In addition, it was suggested to be utilized for commercial applications, e.g. to enhance yield in barley and other species. To produce inoculum, S. indica is mostly cultivated in a complex Hill-Kafer medium (CM medium), however, growth in this medium is slow, and yield of chlamydospores, which are often used for plant root inoculation, is relatively low. RESULTS: We tested and optimized a simple vegetable juice-based medium for an enhanced yield of fungal inoculum. The described vegetable juice (VJ) medium is based on commercially available vegetable juice and is easy to prepare. VJ medium was superior to the currently used CM medium with respect to biomass production in liquid medium and hyphal growth on agar plates. Using solid VJ medium supplemented with sucrose (VJS), a high amount of chlamydospores developed already after 8 days of cultivation, producing significantly more spores than on CM medium. Use of VJ medium is not restricted to S. indica, as it also supported growth of two pathogenic fungi often used in plant pathology experiments: the ascomycete Fusarium graminearum, the causal agent of Fusarium head blight disease on wheat and barley, and Verticillium longisporum, the causal agent of verticillium wilt. CONCLUSIONS: The described VJ medium is recommended for streamlined and efficient production of inoculum for the plant endophytic fungus Serendipita indica and might prove superior for the propagation of other fungi for research purposes.
Osman, Mohamed
Stigloher, Christian
Mueller, Martin J
Waller, Frank
eng
England
2020/03/20
Plant Methods. 2020 Mar 16;16:39. doi: 10.1186/s13007-020-00584-7. eCollection 2020.
%0 Journal Article
%1 osman2020improved
%A Osman, M.
%A Stigloher, C.
%A Mueller, M. J.
%A Waller, F.
%D 2020
%J Plant Methods
%K Aspergillus medium myOwn uni_network
%P 39
%R 10.1186/s13007-020-00584-7
%T An improved growth medium for enhanced inoculum production of the plant growth-promoting fungus Serendipita indica
%U https://www.ncbi.nlm.nih.gov/pubmed/32190103https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-020-00584-7.pdf
%V 16
%X BACKGROUND: The plant endophytic fungus Serendipita indica colonizes roots of a wide range of plant species and can enhance growth and stress resistance of these plants. Due to its ease of axenic cultivation and its broad host plant range including the model plant Arabidopsis thaliana and numerous crop plants, it is widely used as a model fungus to study beneficial fungus-root interactions. In addition, it was suggested to be utilized for commercial applications, e.g. to enhance yield in barley and other species. To produce inoculum, S. indica is mostly cultivated in a complex Hill-Kafer medium (CM medium), however, growth in this medium is slow, and yield of chlamydospores, which are often used for plant root inoculation, is relatively low. RESULTS: We tested and optimized a simple vegetable juice-based medium for an enhanced yield of fungal inoculum. The described vegetable juice (VJ) medium is based on commercially available vegetable juice and is easy to prepare. VJ medium was superior to the currently used CM medium with respect to biomass production in liquid medium and hyphal growth on agar plates. Using solid VJ medium supplemented with sucrose (VJS), a high amount of chlamydospores developed already after 8 days of cultivation, producing significantly more spores than on CM medium. Use of VJ medium is not restricted to S. indica, as it also supported growth of two pathogenic fungi often used in plant pathology experiments: the ascomycete Fusarium graminearum, the causal agent of Fusarium head blight disease on wheat and barley, and Verticillium longisporum, the causal agent of verticillium wilt. CONCLUSIONS: The described VJ medium is recommended for streamlined and efficient production of inoculum for the plant endophytic fungus Serendipita indica and might prove superior for the propagation of other fungi for research purposes.
@article{osman2020improved,
abstract = {BACKGROUND: The plant endophytic fungus Serendipita indica colonizes roots of a wide range of plant species and can enhance growth and stress resistance of these plants. Due to its ease of axenic cultivation and its broad host plant range including the model plant Arabidopsis thaliana and numerous crop plants, it is widely used as a model fungus to study beneficial fungus-root interactions. In addition, it was suggested to be utilized for commercial applications, e.g. to enhance yield in barley and other species. To produce inoculum, S. indica is mostly cultivated in a complex Hill-Kafer medium (CM medium), however, growth in this medium is slow, and yield of chlamydospores, which are often used for plant root inoculation, is relatively low. RESULTS: We tested and optimized a simple vegetable juice-based medium for an enhanced yield of fungal inoculum. The described vegetable juice (VJ) medium is based on commercially available vegetable juice and is easy to prepare. VJ medium was superior to the currently used CM medium with respect to biomass production in liquid medium and hyphal growth on agar plates. Using solid VJ medium supplemented with sucrose (VJS), a high amount of chlamydospores developed already after 8 days of cultivation, producing significantly more spores than on CM medium. Use of VJ medium is not restricted to S. indica, as it also supported growth of two pathogenic fungi often used in plant pathology experiments: the ascomycete Fusarium graminearum, the causal agent of Fusarium head blight disease on wheat and barley, and Verticillium longisporum, the causal agent of verticillium wilt. CONCLUSIONS: The described VJ medium is recommended for streamlined and efficient production of inoculum for the plant endophytic fungus Serendipita indica and might prove superior for the propagation of other fungi for research purposes.},
added-at = {2024-02-15T15:08:22.000+0100},
author = {Osman, M. and Stigloher, C. and Mueller, M. J. and Waller, F.},
biburl = {https://www.bibsonomy.org/bibtex/28e906694f922a8355170cba8a0f26a54/jvsi_all},
doi = {10.1186/s13007-020-00584-7},
interhash = {aee4056992d85aa4a7cf4b3aeddbe3a6},
intrahash = {8e906694f922a8355170cba8a0f26a54},
issn = {1746-4811 (Print)
1746-4811 (Electronic)
1746-4811 (Linking)},
journal = {Plant Methods},
keywords = {Aspergillus medium myOwn uni_network},
note = {Osman, Mohamed
Stigloher, Christian
Mueller, Martin J
Waller, Frank
eng
England
2020/03/20
Plant Methods. 2020 Mar 16;16:39. doi: 10.1186/s13007-020-00584-7. eCollection 2020.},
pages = 39,
timestamp = {2024-02-15T15:11:55.000+0100},
title = {An improved growth medium for enhanced inoculum production of the plant growth-promoting fungus Serendipita indica},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/32190103https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-020-00584-7.pdf},
volume = 16,
year = 2020
}