Living organisms are capable of discriminating thermal stimuli from noxious cold to noxious heat. For more than 30 years, it has been known that plant cells respond to cold with a large and transient depolarization. Recently, using transgenic Arabidopsis (Arabidopsis thaliana) expressing the calcium-sensitive protein aequorin, an increase in cytosolic calcium following cold treatment was observed. Applying the patch-clamp technique to Arabidopsis mesophyll protoplasts, we could identify a transient plasma membrane conductance induced by rapid cooling. This cold-induced transient conductance was characterized as an outward rectifying 33 pS nonselective cation channel. The permeability ratio between calcium and cesium was 0.7, pointing to a permeation pore.3.34 angstrom (phi of cesium). Our experiments thus provide direct evidence for the predicted but not yet measured cold-activated calcium-permeable channel in plants.
%0 Journal Article
%1 RN1153
%A Carpaneto, A.
%A Ivashikina, N.
%A Levchenko, V.
%A Krol, E.
%A Jeworutzki, E.
%A Zhu, J. K.
%A Hedrich, R.
%D 2007
%J Plant Physiology
%K changes myOwn potential transmembrane
%N 1
%P 487-494
%R 10.1104/pp.106.090928
%T Cold transiently activates calcium-permeable channels in Arabidopsis mesophyll cells
%U /brokenurl#<Go to ISI>://WOS:000243350600043
%V 143
%X Living organisms are capable of discriminating thermal stimuli from noxious cold to noxious heat. For more than 30 years, it has been known that plant cells respond to cold with a large and transient depolarization. Recently, using transgenic Arabidopsis (Arabidopsis thaliana) expressing the calcium-sensitive protein aequorin, an increase in cytosolic calcium following cold treatment was observed. Applying the patch-clamp technique to Arabidopsis mesophyll protoplasts, we could identify a transient plasma membrane conductance induced by rapid cooling. This cold-induced transient conductance was characterized as an outward rectifying 33 pS nonselective cation channel. The permeability ratio between calcium and cesium was 0.7, pointing to a permeation pore.3.34 angstrom (phi of cesium). Our experiments thus provide direct evidence for the predicted but not yet measured cold-activated calcium-permeable channel in plants.
@article{RN1153,
abstract = {Living organisms are capable of discriminating thermal stimuli from noxious cold to noxious heat. For more than 30 years, it has been known that plant cells respond to cold with a large and transient depolarization. Recently, using transgenic Arabidopsis (Arabidopsis thaliana) expressing the calcium-sensitive protein aequorin, an increase in cytosolic calcium following cold treatment was observed. Applying the patch-clamp technique to Arabidopsis mesophyll protoplasts, we could identify a transient plasma membrane conductance induced by rapid cooling. This cold-induced transient conductance was characterized as an outward rectifying 33 pS nonselective cation channel. The permeability ratio between calcium and cesium was 0.7, pointing to a permeation pore.3.34 angstrom (phi of cesium). Our experiments thus provide direct evidence for the predicted but not yet measured cold-activated calcium-permeable channel in plants.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Carpaneto, A. and Ivashikina, N. and Levchenko, V. and Krol, E. and Jeworutzki, E. and Zhu, J. K. and Hedrich, R.},
biburl = {https://www.bibsonomy.org/bibtex/28fabad11a68111163c206930899094c6/rainerhedrich_2},
doi = {10.1104/pp.106.090928},
interhash = {db10693de2bb7aa4dbf4db93d9299872},
intrahash = {8fabad11a68111163c206930899094c6},
issn = {0032-0889},
journal = {Plant Physiology},
keywords = {changes myOwn potential transmembrane},
note = {124ep
Times Cited:82
Cited References Count:42},
number = 1,
pages = {487-494},
timestamp = {2024-02-14T14:38:32.000+0100},
title = {Cold transiently activates calcium-permeable channels in Arabidopsis mesophyll cells},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000243350600043},
volume = 143,
year = 2007
}